Cytotoxic activity and structural features of Ru(II)/phosphine/amino acid complexes.

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Autor(es): dc.creatorSantos, Edjane Rocha dos-
Autor(es): dc.creatorGraminha, Angelica Ellen-
Autor(es): dc.creatorSchultz, Mario Sergio-
Autor(es): dc.creatorCorreia, Isabel-
Autor(es): dc.creatorAraujo, Heloisa Sobreiro Selistre de-
Autor(es): dc.creatorCorrea, Rodrigo de Souza-
Autor(es): dc.creatorEllena, Javier Alcides-
Autor(es): dc.creatorLacerda, Elisângela de Paula Silveira-
Autor(es): dc.creatorPessoa, João Costa-
Autor(es): dc.creatorBatista, Alzir Azevedo-
Data de aceite: dc.date.accessioned2025-08-21T15:29:09Z-
Data de disponibilização: dc.date.available2025-08-21T15:29:09Z-
Data de envio: dc.date.issued2018-10-16-
Data de envio: dc.date.issued2018-10-16-
Data de envio: dc.date.issued2018-
Fonte completa do material: dc.identifierhttp://www.repositorio.ufop.br/handle/123456789/10377-
Fonte completa do material: dc.identifierhttps://www.sciencedirect.com/science/article/pii/S016201341730510X#!-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1016389-
Descrição: dc.descriptionThirteen new ruthenium amino acid complexes were synthesized and characterized. They were obtained by the reaction of α-amino acids (AA) with [RuCl2(P-P)(N-N)], where P-P = 1,4-bis(diphenylphosphino)butane (dppb) or 1,3-bis(diphenylphosphino)propane (dppp) and N-N = 4,4′-dimethyl-2,2′-bipyridine (4′-Mebipy), 5,5′-dimethyl-2,2′-bipyridine (5′-Mebipy) or 4,4′-Methoxy-2-2′-bipyridine (4′-MeObipy). This afforded a family of complexes formulated as [Ru(AA-H)(P-P)(N-N)]PF6, where AA = glycine (Gly), L-alanine (Ala), L-valine (Val), L-tyrosine (Tyr), L-tryptophan (Trp), L-histidine (His) and L-methionine (Met). All compounds were characterized by elemental analysis, spectroscopic and electrochemical techniques. The [Ru(AA-H)(P-P)(N-N)]PF6 complexes are octahedral (the AA-H ligand binding involves N-amine and O-carboxylate), diamagnetic (low-spin d6, S = 0) and present bands due to electronic transitions in the visible region. 1H, 13C{1H} and 31P{1H} NMR spectra of the complexes indicate the presence of C2 symmetry, and the identification of diastereoisomers. In vitro cytotoxicity assays of the compounds and cisplatin were carried out using MDA-MB-231 (human breast) tumor cell line and a non-tumor breast cell line (MCF-10A). Most complexes present promising results with IC50 values comparable with the reference drug cisplatin and high selectivity indexes were found for the complexes containing L-Trp. The binding of two Ru-precursors of the type [RuCl2(dppb)(NN)] (N-N = 4′-MeObipy or 4′-Mebipy) to the blood transporter protein human serum albumin (HSA) was evaluated by fluorescence and circular dichroism spectroscopy. Both complexes bind HSA, probably in the hydrophobic pocket near Trp214, and the Ru-complex containing 4′-MeObipy shows higher affinity for HSA than the 4′-Mebipy one.-
Formato: dc.formatapplication/pdf-
Idioma: dc.languageen-
Direitos: dc.rightsrestrito-
Palavras-chave: dc.subjectRuthenium complexes-
Palavras-chave: dc.subjectHuman serum albumin-
Palavras-chave: dc.subjectCircular dichroism-
Título: dc.titleCytotoxic activity and structural features of Ru(II)/phosphine/amino acid complexes.-
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