Comparative study of a novel lateral flow rapid test with conventional serological test systems for the diagnosis of canine leishmaniosis in Croatia and Brazil.

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Autor(es): dc.creatorMahdavi, Rouzbeh-
Autor(es): dc.creatorMartinkovic, Franjo-
Autor(es): dc.creatorShams-Eldin, Hosam-
Autor(es): dc.creatorPereira, Ingrid Estevam-
Autor(es): dc.creatorReis, Alexandre Barbosa-
Autor(es): dc.creatorLatz, Andreas-
Autor(es): dc.creatorHeinz, Daniela-
Autor(es): dc.creatorAira, Cristina P.-
Autor(es): dc.creatorFresco Taboada, Alba-
Autor(es): dc.creatorAbass, Elfadil-
Autor(es): dc.creatorRomero Olmedo, Jelena-
Autor(es): dc.creatorTeixeira, Henrique Couto-
Autor(es): dc.creatorUlrich, Steinhoff-
Data de aceite: dc.date.accessioned2025-08-21T15:25:00Z-
Data de disponibilização: dc.date.available2025-08-21T15:25:00Z-
Data de envio: dc.date.issued2025-02-06-
Data de envio: dc.date.issued2025-02-06-
Data de envio: dc.date.issued2023-
Fonte completa do material: dc.identifierhttps://www.repositorio.ufop.br/handle/123456789/19728-
Fonte completa do material: dc.identifierhttps://doi.org/10.3390/pathogens13020109-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1013926-
Descrição: dc.descriptionControl of canine infections with Leishmania infantum (L. infantum), a major zoonotic disease in Brazil and southern Europe, is becoming increasingly important due to its close proximity to humans, the increasing import of dogs from endemic regions and the impact of climate change on vector spreading. Simple, rapid and reliable diagnostic tests are therefore needed to detect infected dogs. Here, we re-evaluated different serological methods for the diagnosis of canine leishmaniosis (CanL) in Croatia and Brazil. The diagnostic performance of the indirect fluorescent antibody test (IFAT) and the VetLine® Leishmania ELISA (GSD Frankfurt, Germany) was compared with three rKLi8.3-based diagnostic test systems, the rKLi8.3 ELISA (GSD Frankfurt, Germany), the INgezim® Leishma CROM (GSD Madrid, Spain) lateral flow test (LFT) and the VetBlot® Leishmania LineBlot (GSD Frankfurt, Germany). CanL symptomatic dogs were efficiently diagnosed by all tests, except the VetLine® Leishmania ELISA, which is based on whole Leishmania antigens. The advantage of rKLi8.3 was also observed in oligo- and asymptomatic dogs from Brazil and Croatia, although with reduced diagnostic efficiency compared to symptomatic dogs. Similar to IFAT and rKLi8.3 ELISA, the LFT did not cross-react with other common canine pathogens; it showed very high specificity for healthy dogs from endemic regions in both countries and did not react with healthy, vaccinated dogs in Brazil. In conclusion, serodiagnostic tests based on the rKLi8.3 antigens are superior to whole parasite antigens, and the LFT has the advantage of providing a laboratory-independent, rapid and specific diagnosis of CanL.-
Formato: dc.formatapplication/pdf-
Idioma: dc.languageen-
Direitos: dc.rightsaberto-
Direitos: dc.rightsThis article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). Fonte: PDF do artigo.-
Palavras-chave: dc.subjectCanine leishmaniosis-
Palavras-chave: dc.subjectPOC diagnostics for leishmaniosis-
Palavras-chave: dc.subjectLateral flow test-
Palavras-chave: dc.subjectLine blot-
Título: dc.titleComparative study of a novel lateral flow rapid test with conventional serological test systems for the diagnosis of canine leishmaniosis in Croatia and Brazil.-
Aparece nas coleções:Repositório Institucional - UFOP

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