Intracellular signal triggered by cholera toxin in Saccharomyces boulardii and Saccharomyces cerevisiae.

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Autor(es): dc.creatorBrandão, Rogélio Lopes-
Autor(es): dc.creatorCastro, Ieso de Miranda-
Autor(es): dc.creatorBambirra, Eduardo Alves-
Autor(es): dc.creatorAmaral, Sheila Coutinho-
Autor(es): dc.creatorFietto, Luciano Gomes-
Autor(es): dc.creatorTrópia, Maria José Magalhães-
Autor(es): dc.creatorNeves, Maria José-
Autor(es): dc.creatorSantos, Raquel Gouvêa dos-
Autor(es): dc.creatorGomes, Newton Carlos Marcial-
Autor(es): dc.creatorNicoli, Jacques Robert-
Data de aceite: dc.date.accessioned2025-08-21T15:22:15Z-
Data de disponibilização: dc.date.available2025-08-21T15:22:15Z-
Data de envio: dc.date.issued2017-03-20-
Data de envio: dc.date.issued2017-03-20-
Data de envio: dc.date.issued1998-
Fonte completa do material: dc.identifierhttp://www.repositorio.ufop.br/handle/123456789/7394-
Fonte completa do material: dc.identifierhttp://aem.asm.org/content/64/2/564.long-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1012191-
Descrição: dc.descriptionAs is the case for Saccharomyces boulardii, Saccharomyces cerevisiae W303 protects Fisher rats against cholera toxin (CT). The addition of glucose or dinitrophenol to cells of S. boulardii grown on a nonfermentable carbon source activated trehalase in a manner similar to that observed for S. cerevisiae. The addition of CT to the same cells also resulted in trehalase activation. Experiments performed separately on the A and B subunits of CT showed that both are necessary for activation. Similarly, the addition of CT but not of its separate subunits led to a cyclic AMP (cAMP) signal in both S. boulardii and S. cerevisiae. These data suggest that trehalase stimulation by CT probably occurred through the cAMP-mediated protein phosphorylation cascade. The requirement of CT subunit B for both the cAMP signal and trehalase activation indicates the presence of a specific receptor on the yeasts able to bind to the toxin, a situation similar to that observed for mammalian cells. This hypothesis was reinforced by experiments with 125I-labeled CT showing specific binding of the toxin to yeast cells. The adhesion of CT to a receptor on the yeast surface through the B subunit and internalization of the A subunit (necessary for the cAMP signal and trehalase activation) could be one more mechanism explaining protection against the toxin observed for rats treated with yeasts.-
Formato: dc.formatapplication/pdf-
Idioma: dc.languageen-
Direitos: dc.rightsrestrito-
Título: dc.titleIntracellular signal triggered by cholera toxin in Saccharomyces boulardii and Saccharomyces cerevisiae.-
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