Plasma membrane proteomes of differentially matured dendritic cells identified by LC MS/MS combined with iTRAQ labelling.

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Autor(es): dc.creatorBernard, Stéphanie Ferret-
Autor(es): dc.creatorBorges, William de Castro-
Autor(es): dc.creatorDowle, Adam A.-
Autor(es): dc.creatorSanin, David E.-
Autor(es): dc.creatorCook, Peter C.-
Autor(es): dc.creatorTurner, Joseph D.-
Autor(es): dc.creatorMacDonald, Andrew S.-
Autor(es): dc.creatorThomas, Jerry R.-
Autor(es): dc.creatorMountford, Adrian P.-
Data de aceite: dc.date.accessioned2025-08-21T15:21:46Z-
Data de disponibilização: dc.date.available2025-08-21T15:21:46Z-
Data de envio: dc.date.issued2017-07-03-
Data de envio: dc.date.issued2017-07-03-
Data de envio: dc.date.issued2011-
Fonte completa do material: dc.identifierhttp://www.repositorio.ufop.br/handle/123456789/8136-
Fonte completa do material: dc.identifierhttps://doi.org/10.1016/j.jprot.2011.10.010-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1011874-
Descrição: dc.descriptionDendritic cells (DCs) play a pivotal role in polarising Th lymphocyte subsets but it is unclear whatmolecular events occur when DCs generate Th2-type responses. Here, we analysed plasma membrane-enriched fractions from immature, pro-Th1 and pro-Th2 DCs and used a combination of iTRAQ labelling and LC–MS/MS to quantify changes in the proteomes. Analysis was performed on triplicate biological samples and changes verified by flow cytometry. MHC class II molecules and CD29 were up-regulated in pro-Th1 DCs whilst CD18 and CD44 were upregulated in pro-Th2 DCs. One of the most down-regulated molecules in pro-Th1 DCs was YM-1 whilst the greatest decrease in pro-Th2 DCs was NAP-22. Other molecules upregulated in pro-Th2 DC compared to pro-Th1 DCs included some potentially involved in protein folding during antigen processing (clathrin and Rab-7), whilst other non-membrane proteins such as enzymes/transporters related to cell metabolism(malate dehydrogenase, pyruvate kinase, and ATPase Na+/K+) were also recorded. This suggests that pro-Th2 DCs are more metabolically active while pro-Th1 DCs have a mature ‘end state’. Overall, althoughseveralmolecules were preferentially expressed on pro-Th2 DCs, our proteomics data support the view of a ‘limited maturation’ of pro-Th2 DCs compared to pro-Th1 DCs.-
Formato: dc.formatapplication/pdf-
Idioma: dc.languageen-
Direitos: dc.rightsaberto-
Direitos: dc.rightsEste artigo está sob licença Creative Commons que permite copiar, distribuir e transmitir o trabalho desde que sejam citados o autor e o licenciante. Fonte: o próprio artigo.-
Palavras-chave: dc.subjectParasitic helminth-
Título: dc.titlePlasma membrane proteomes of differentially matured dendritic cells identified by LC MS/MS combined with iTRAQ labelling.-
Aparece nas coleções:Repositório Institucional - UFOP

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