Impedimetric immunosensor for rapid and simultaneous detection of chagas and visceral leishmaniasis for point of care diagnosis.

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MetadadosDescriçãoIdioma
Autor(es): dc.creatorCordeiro, Taís Aparecida Reis-
Autor(es): dc.creatorMartins, Helen Rodrigues-
Autor(es): dc.creatorFranco, Diego Leoni-
Autor(es): dc.creatorSantos, Fred Luciano Neves-
Autor(es): dc.creatorCeledon, Paola Alejandra Fiorani-
Autor(es): dc.creatorCantuária, Vinícius Lopes-
Autor(es): dc.creatorLana, Marta de-
Autor(es): dc.creatorReis, Alexandre Barbosa-
Autor(es): dc.creatorFerreira, Lucas Franco-
Data de aceite: dc.date.accessioned2025-08-21T15:21:23Z-
Data de disponibilização: dc.date.available2025-08-21T15:21:23Z-
Data de envio: dc.date.issued2021-09-03-
Data de envio: dc.date.issued2021-09-03-
Data de envio: dc.date.issued2019-
Fonte completa do material: dc.identifierhttp://www.repositorio.ufop.br/jspui/handle/123456789/13641-
Fonte completa do material: dc.identifierhttps://www.sciencedirect.com/science/article/abs/pii/S0956566320305650?via%3Dihub-
Fonte completa do material: dc.identifierhttps://doi.org/10.1016/j.bios.2020.112573-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1011618-
Descrição: dc.descriptionIn this work, a dual detection system based on an impedimetric immunosensor was developed for the first time for the simultaneous detection of anti-Trypanosoma cruzi and anti-Leishmania infantum antibodies in human and dog serum samples. The IBMP 8.1 and rLci1A/rLci2B recombinant antigens were immobilized over the surface of dual screen-printed carbon electrodes (W1 and W2) modified with poly (4-hydroxyphenylacetic acid). Under optimized conditions, the immunosensor recognized specific interactions for anti-T. cruzi antibodies up to a dilution of 1:10,240 and for anti-L. infantum up to 1:5120 in canine serum samples. Relative standard deviation (RSD) values of 2.8% for W1 and 3.6% for W2 were obtained for T. cruzi (W1) and L. infantum antigen (W2) samples in three different electrodes for 3 days (n = 9). The immunosensor was stored at 4 ◦C for 8 weeks, with activity retention of 70.2% in W1 and 78.2% in W2. The results using the recombinant proteins revealed that all antigens discriminated between negative and positive samples (p < 0.0001) in both dog and human groups, as well as no cross-reactivity could be detected among sera with other infections. With this approach, immunosensor-based diagnostic tests achieved 100% accuracy, suggesting that the antigens are eligible to enter Phase-II studies.-
Formato: dc.formatapplication/pdf-
Idioma: dc.languageen-
Direitos: dc.rightsrestrito-
Palavras-chave: dc.subjectDual detection-
Palavras-chave: dc.subjectElectropolymerization-
Título: dc.titleImpedimetric immunosensor for rapid and simultaneous detection of chagas and visceral leishmaniasis for point of care diagnosis.-
Aparece nas coleções:Repositório Institucional - UFOP

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