Antiplasmodial activity and cytotoxicity, isolation of active alkaloids, and dereplication of Xylopia sericea leaves ethanol extract by UPLC-DAD-ESI-MS/MS.

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.creatorGontijo, Douglas da Costa-
Autor(es): dc.creatorBrandão, Geraldo Célio-
Autor(es): dc.creatorNascimento, Maria Fernanda Alves do-
Autor(es): dc.creatorOliveira, Alaide Braga de-
Data de aceite: dc.date.accessioned2025-08-21T15:19:00Z-
Data de disponibilização: dc.date.available2025-08-21T15:19:00Z-
Data de envio: dc.date.issued2019-04-15-
Data de envio: dc.date.issued2019-04-15-
Data de envio: dc.date.issued2019-
Fonte completa do material: dc.identifierhttp://www.repositorio.ufop.br/handle/123456789/11021-
Fonte completa do material: dc.identifierhttps://onlinelibrary.wiley.com/doi/full/10.1111/jphp.13029-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1010198-
Descrição: dc.descriptionObjectives: To assess the antiplasmodial activity of the ethanol extract of Xylopia sericea leaves, Annonaceae, often associated with antimalarial use and to perform a bioguided isolation of active compounds. Methods: Dereplication of ethanol extract by the UPLC-DAD-ESI-MS/MS technique allowed the identification of the major constituents, isolation and identification of alkaloids. The antiplasmodial and cytotoxic activity of the extract, fractions and isolated compounds was evaluated against the chloroquine-resistant W2 strain Plasmodium falciparum and HepG2 cells, respectively. Key findings: Ethanol extract showed high reduction of parasitemia as well as moderate cytotoxicity (86.5 3.0% growth inhibition at 50 lg/ml and CC50 72.1 5.1 lg/ml, respectively). A total of eight flavonoids were identified, and two aporphine alkaloids, anonaine and O-methylmoschatoline, were isolated. Anonaine disclosed significant antiplasmodial effect and moderate cytotoxicity (IC50 23.2 2.7 lg/ml, CC50 38.3 2.3 lg/ml, SI 1.6) while O-methylmoschatoline was not active against P. falciparum and showed a low cytotoxicity (33.5 1.9% growth inhibition at 50 lg/ml, CC50 274.4 0.5 lg/ml). Conclusions: Characterization of Xylopia sericea leaves ethanol extract by UPLCDAD-ESI-MS/MS as well as its antiplasmodial activity and the occurrence of anonaine and O-methylmoschatoline in this Xylopia species are reported by the first time.-
Formato: dc.formatapplication/pdf-
Idioma: dc.languageen-
Direitos: dc.rightsrestrito-
Palavras-chave: dc.subjectExtract dereplication-
Palavras-chave: dc.subjectFlavonoids-
Título: dc.titleAntiplasmodial activity and cytotoxicity, isolation of active alkaloids, and dereplication of Xylopia sericea leaves ethanol extract by UPLC-DAD-ESI-MS/MS.-
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