Method for the determination of benzene metabolite t,t-Muconic acid in urine by HPLC-UV with an Ion exclusion column.

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.creatorGomes, Rafaela de Paiva-
Autor(es): dc.creatorSanson, Ananda Lima-
Autor(es): dc.creatorLobo, Fabiana Aparecida-
Autor(es): dc.creatorAfonso, Robson José de Cássia Franco-
Autor(es): dc.creatorCoutrim, Maurício Xavier-
Data de aceite: dc.date.accessioned2025-08-21T15:08:58Z-
Data de disponibilização: dc.date.available2025-08-21T15:08:58Z-
Data de envio: dc.date.issued2018-03-13-
Data de envio: dc.date.issued2018-03-13-
Data de envio: dc.date.issued2016-
Fonte completa do material: dc.identifierhttp://www.repositorio.ufop.br/handle/123456789/9607-
Fonte completa do material: dc.identifierhttps://doi.org/10.3390/separations3020014-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1003545-
Descrição: dc.descriptionThe benzene metabolite trans,trans-muconic acid (tt-MA) is widely used as a biological indicator of exposure to this xenobiotic. An analytical method was developed and validated for the determination of urinary tt-MA using solid phase extraction onto a Strata SAX cartridge. The analysis was performed by HPLC with an Aminex HPX-87H ion exclusion column and a UV diode array detector. The sample preparation conditions were optimized using a 24 1 factorial design. Comparison of the slopes of standard analytical curves prepared in aqueous solution and urine showed that the biological matrix suppressed the tt-MA signal by around 50%, so the analytical curves were prepared with tt-MA standard at low concentrations in pooled urine. The analytical curves in the range of 5–500 g L 1 showed determination coefficients values (R2) > 0.99 for tt-MA standard in water and R2 > 0.98 for tt-MA standards in pooled urine. The coefficients of variation obtained using seven replicates were lower than 3.6%, and recoveries of tt-MA from solutions containing 5, 25, 50 and 100 g L 1 of the analyte were in the range 85%–90%, demonstrating the satisfactory precision and accuracy of the method. The limits of detection and quantification were 0.11 and 0.36 g L 1, respectively. The benefits of this new method developed is the possibility of complete chromatographic peak separation for the determination of tt-MA at baseline, without matrix components’ interference as normally found in the C18 column. This is the first time that this chromatographic column has been used for the analysis of tt-MA in urine.-
Formato: dc.formatapplication/pdf-
Idioma: dc.languageen-
Direitos: dc.rightsaberto-
Direitos: dc.rightsThis article is an open access article distributed under the terms and conditions of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/). Fonte: o próprio artigo.-
Palavras-chave: dc.subjectExposure biomarker-
Palavras-chave: dc.subjectEnvironmental exposure-
Palavras-chave: dc.subjectBenzene-
Título: dc.titleMethod for the determination of benzene metabolite t,t-Muconic acid in urine by HPLC-UV with an Ion exclusion column.-
Aparece nas coleções:Repositório Institucional - UFOP

Não existem arquivos associados a este item.