A specific Leishmania infantum polyepitope vaccine triggers Th1-type immune response and protects against experimental visceral leishmaniasis.

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Autor(es): dc.creatorOstolin, Thais Lopes Valentim Di Paschoale-
Autor(es): dc.creatorGusmão, Miriã Rodrigues-
Autor(es): dc.creatorMathias, Fernando Augusto Siqueira-
Autor(es): dc.creatorCardoso, Jamille Mirelle de Oliveira-
Autor(es): dc.creatorRoatt, Bruno Mendes-
Autor(es): dc.creatorSoares, Rodrigo Dian de Oliveira Aguiar-
Autor(es): dc.creatorRuiz, Jeronimo Conceição-
Autor(es): dc.creatorResende, Daniela de Melo-
Autor(es): dc.creatorBrito, Rory Cristiane Fortes de-
Autor(es): dc.creatorReis, Alexandre Barbosa-
Data de aceite: dc.date.accessioned2025-08-21T15:06:16Z-
Data de disponibilização: dc.date.available2025-08-21T15:06:16Z-
Data de envio: dc.date.issued2023-10-06-
Data de envio: dc.date.issued2023-10-06-
Data de envio: dc.date.issued2021-
Fonte completa do material: dc.identifierhttp://www.repositorio.ufop.br/jspui/handle/123456789/17535-
Fonte completa do material: dc.identifierhttps://www.sciencedirect.com/science/article/pii/S0008874922001174?via%3Dihub-
Fonte completa do material: dc.identifierhttps://doi.org/10.1016/j.cellimm.2022.104592-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1002479-
Descrição: dc.descriptionThe development of an immunogenic, effective, and safe vaccine is essential as an alternative for disease control. The present study aimed to evaluate the immunogenicity and efficacy potential of a polyepitope T-cell antigen candidate against visceral leishmaniasis in a murine model. BALB/c mice were immunized with three doses subcutaneously with Poly-T Leish alone or adjuvanted with Saponin plus Monophosphoryl lipid A, with 15-day intervals between doses, and challenged with 107 stationary-phase Leishmania infantum promastigotes via tail vein. Immunogenicity and parasitism in spleen and liver of immunized mice were evaluated 45 days postchallenge. Our results revealed that the immunization with Poly-T Leish and Poly-T Leish/SM increases the percentage of specific T (CD4+ and CD8+) lymphocytes proliferation in vitro after antigen-specific stimulation. Also, Poly-T Leish and Poly-T Leish/SM groups showed a high percentage of IFN-γ and TNF-α-producing T cells, meanwhile, the Poly-T Leish/SM group also showed an increased percentage of multifunctional T cells producing double and triple-positive (IFN-γ+TNF-α+IL-2+) cytokines. The immunization with Poly-T Leish or Poly-T Leish/ SM stimulated a decreased IL-4 and IL-10 compared to the Saline and adjuvant group. Poly-T Leish/SM immunized mice exhibit a noteworthy reduction in the parasite burden (spleen and liver) through real-time PCR (96%). Moreover, we observed higher nitrite secretion in 120-hour stimulated-culture supernatant using Griess method. We demonstrated that the Poly-T Leish/SM candidate was potentially immunogenic, providing enhancement of protective immune mechanisms, and conferred protection reducing parasitism. Our candidate was considered potential against visceral leishmaniasis, and eventually, could be tested in phase I and II clinical trials in dogs.-
Formato: dc.formatapplication/pdf-
Idioma: dc.languageen-
Direitos: dc.rightsrestrito-
Palavras-chave: dc.subjectVisceral leishmaniasis-
Palavras-chave: dc.subjectLeishmania infantum-
Palavras-chave: dc.subjectProtozoan proteins-
Palavras-chave: dc.subjectPolyepitope vaccine-
Palavras-chave: dc.subjectImmunogenicity-
Título: dc.titleA specific Leishmania infantum polyepitope vaccine triggers Th1-type immune response and protects against experimental visceral leishmaniasis.-
Aparece nas coleções:Repositório Institucional - UFOP

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