Detection of glycidic receptors in microalgae using glycodendrons as probes : a new tool for studies on cell surface interactions.

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MetadadosDescriçãoIdioma
Autor(es): dc.creatorGonçalves, Cíntia de Almeida-
Autor(es): dc.creatorFigueiredo, Rute Cunha-
Autor(es): dc.creatorGiani, Alessandra-
Autor(es): dc.creatorCollado, Daniel-
Autor(es): dc.creatorPérez-Inestrosa, Ezequiel-
Autor(es): dc.creatorRojo Marcos, Francisco Javier-
Autor(es): dc.creatorFigueredo, Cleber Cunha-
Data de aceite: dc.date.accessioned2025-08-21T15:04:13Z-
Data de disponibilização: dc.date.available2025-08-21T15:04:13Z-
Data de envio: dc.date.issued2019-05-08-
Data de envio: dc.date.issued2019-05-08-
Data de envio: dc.date.issued2019-
Fonte completa do material: dc.identifierhttp://www.repositorio.ufop.br/handle/123456789/11234-
Fonte completa do material: dc.identifierhttps://link.springer.com/article/10.1007%2Fs10811-018-1555-6-
Fonte completa do material: dc.identifierhttps://doi.org/10.1007/s10811-018-1555-6-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1001887-
Descrição: dc.descriptionCell recognition, adhesion, and internalization are involved in infectious, reproductive, and inflammatory processes and are generally mediated by interactions between molecules located in the cell membrane and the extracellular matrix. These processes can decrease proliferation rates and they are well known for bacteria, fungi, and animals, but there is a lack of knowledge regarding autotrophic cells. Carbohydrates and proteins (e.g., lectins) are important molecules for cell interactions and information about these molecules is essential to better understand many biological phenomena in uni- or multicellular organisms. Most studies focus on the identification of the carbohydrates present on the cell surface by using labeled lectins. Alternatively, here we present a pioneer research performed by using three different labeled carbohydrates in a multivalent presentation (glycodendrons) to detect the presence of carbohydrate receptors (e.g., lectins) on cell surfaces of 12 algal species. The goal of this study was to detect some specificity in these molecular interactions, but in a reverse way in comparison to that commonly described in the literature. We tested trivalent molecules containing residuals of D-mannose, L-fucose, or N-acetyl-galactosamine to identify their bindings with the corresponding lectins expressed on cell surfaces. We envisage that our new approach could be an alternative tool for taxonomic and physiological studies on microalgae or even on other groups of organisms. Based on our results, the receptors found in the cell surface of the algal species tend to differ in composition, quantity, and distribution. The differences were mainly species-specific, since no patterns were identified at higher taxonomic level. Moreover, like lectins, labeled carbohydrates were proved to be a reliable tool for the study of cell surface composition.-
Formato: dc.formatapplication/pdf-
Idioma: dc.languageen-
Direitos: dc.rightsrestrito-
Palavras-chave: dc.subjectLectins-
Palavras-chave: dc.subjectCarbohydrates-
Palavras-chave: dc.subjectFluorescent probes-
Palavras-chave: dc.subjectCell recognition-
Palavras-chave: dc.subjectAlgae-
Título: dc.titleDetection of glycidic receptors in microalgae using glycodendrons as probes : a new tool for studies on cell surface interactions.-
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