eNOS T-786C polymorphism affects atorvastatin-induced changes in erythrocyte membrane fluidity

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorNagassaki, S.-
Autor(es): dc.creatorHerculano, R. D.-
Autor(es): dc.creatorGraeff, Carlos Frederico de Oliveira-
Autor(es): dc.creatorTanus-Santos, J. E.-
Data de aceite: dc.date.accessioned2021-03-10T16:47:46Z-
Data de disponibilização: dc.date.available2021-03-10T16:47:46Z-
Data de envio: dc.date.issued2014-05-20-
Data de envio: dc.date.issued2014-05-20-
Data de envio: dc.date.issued2009-04-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s00228-008-0602-7-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/8438-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/8438-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionStatins have pleiotropic effects, including endothelial nitric oxide synthase (eNOS) upregulation and increased nitric oxide formation, which can be modulated by a genetic polymorphism in the promoter region of the eNOS gene (T-786C). Here, we report our investigation of whether this polymorphism modulates the effects of atorvastatin on the fluidity of erythrocyte membranes.We genotyped 200 healthy subjects (males, 18-60 years of age) and then randomly selected 15 of these with the TT genotype and 15 with the CC genotype to receive placebo or atorvastatin (10 mg/day oral administration) for 14 days. Cell membrane fluidity was evaluated by electron paramagnetic resonance (EPR) and spin-labeling method. The EPR spectra were registered on a VARIAN-E4 spectrometer. Thiobarbituric acid-reactive species (TBA-RS) and plasma membrane cholesterol were determined in the erythrocytes.Atorvastatin reduced membrane fluidity in CC subjects (P < 0.05) but not in those with the TT genotype (P > 0.05). While no significant differences were found in plasma membrane cholesterol concentrations, higher TBA-RS concentrations were found in the CC subjects than in the TT subjects (P < 0.05).These findings suggest that a short treatment with atorvastatin is disadvantageous to subjects with the CC genotype for the T-786C polymorphism compared to those with TT genotype, at least in terms of the hemorheological properties of erythrocytes.-
Formato: dc.format385-392-
Idioma: dc.languageen-
Publicador: dc.publisherSpringer-
Relação: dc.relationEuropean Journal of Clinical Pharmacology-
Relação: dc.relation2.679-
Relação: dc.relation1,159-
Direitos: dc.rightsclosedAccess-
Palavras-chave: dc.subjectEndothelial nitric oxide synthase-
Palavras-chave: dc.subjectEPR-
Palavras-chave: dc.subjectGenotypes-
Palavras-chave: dc.subjectNitric oxide-
Palavras-chave: dc.subjectPolymorphisms-
Palavras-chave: dc.subjectStatins-
Título: dc.titleeNOS T-786C polymorphism affects atorvastatin-induced changes in erythrocyte membrane fluidity-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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