Development and validation of an analytical method by RP-HPLC for quantification of sibutramine hydrochloride in pharmaceutical capsules

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorChorilli, Marlus-
Autor(es): dc.creatorBonfilio, Rudy-
Autor(es): dc.creatorChicarelli, Renata da Silva-
Autor(es): dc.creatorSalgado, Hérida Regina Nunes-
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Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionPrograma de Apoio ao Desenvolvimento Científico da Faculdade de Ciências Farmacêuticas da UNESP (PADC)-
Descrição: dc.descriptionSibutramine is a monoamine re-uptake inhibitor used for the treatment of obesity. In the present study a rapid, sensitive and economical HPLC method was developed and fully validated for analysis of sibutramine HCl in pharmaceutical capsules. HPLC analyses were carried out by using an isocratic elution mode with a mobile phase constituted by sodium phosphate buffer (pH 2.5) and methanol (30 : 70, v/v), flow rate at 1.0 mL min(-1), column temperature at 40 degrees C, UV detection wavelength at 225 nm and 20 mu L of injection volume. The validation parameters were in accordance with FDA and ICH specifications, showing accuracy, precision, selectivity, robustness and linearity from 4.5 to 19.5 mg L(-1) of sibutramine HCl. The limits of detection and quantification were 0.666 and 2.018 mg L(-1), respectively. The validated method is suitable for quality control applications and its advantages over the already existing methods are simplicity and reduced analysis time.-
Formato: dc.format985-990-
Idioma: dc.languageen-
Publicador: dc.publisherRoyal Soc Chemistry-
Relação: dc.relationAnalytical Methods-
Relação: dc.relation2.073-
Relação: dc.relation0,619-
Direitos: dc.rightsclosedAccess-
Título: dc.titleDevelopment and validation of an analytical method by RP-HPLC for quantification of sibutramine hydrochloride in pharmaceutical capsules-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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