Heteroplasmy in Hair: Study of Mitochondrial DNA Third Hypervariable Region in Hair and Blood Samples

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorPaneto, Greiciane G.-
Autor(es): dc.creatorLongo, Larissa V. G.-
Autor(es): dc.creatorMartins, Joyce A.-
Autor(es): dc.creatorde Camargo, Maria Angelica-
Autor(es): dc.creatorCosta, Jeane C.-
Autor(es): dc.creatorde Mello, Aline C. O.-
Autor(es): dc.creatorChen, Bety-
Autor(es): dc.creatorOliveira, Rogerio N.-
Autor(es): dc.creatorHirata, Mario H.-
Autor(es): dc.creatorCicarelli, Regina Maria Barretto-
Data de aceite: dc.date.accessioned2021-03-10T16:46:43Z-
Data de disponibilização: dc.date.available2021-03-10T16:46:43Z-
Data de envio: dc.date.issued2014-05-20-
Data de envio: dc.date.issued2014-05-20-
Data de envio: dc.date.issued2010-05-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1111/j.1556-4029.2010.01339.x-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/7886-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/7886-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionProcesso FAPESP: 05/03984-5-
Descrição: dc.descriptionProcesso FAPESP: 06/06772-1-
Descrição: dc.descriptionCNPq: 2007-0/140052-
Descrição: dc.descriptionMitochondrial DNA (mtDNA) analysis has proved useful for forensic identification especially in cases where nuclear DNA is not available, such as with hair evidence. Heteroplasmy, the presence of more than one type of mtDNA in one individual, is a common situation often reported in the first and second mtDNA hypervariable regions (HV1/HV2), particularly in hair samples. However, there is no data about heteroplasmy frequency in the third mtDNA hypervariable region (HV3). To investigate possible heteroplasmy hotspots, HV3 from hair and blood samples of 100 individuals were sequenced and compared. No point heteroplasmy was observed, but length heteroplasmy was, both in C-stretch and CA repeat. To observe which CA "alleles" were present in each tissue, PCR products were cloned and re-sequenced. However, no variation among CA alleles was observed. Regarding forensic practice, we conclude that point heteroplasmy in HV3 is not as frequent as in the HV1/HV2.-
Formato: dc.format715-718-
Idioma: dc.languageen-
Publicador: dc.publisherWiley-Blackwell Publishing, Inc-
Relação: dc.relationJournal of Forensic Sciences-
Relação: dc.relation1.184-
Relação: dc.relation0,686-
Direitos: dc.rightsclosedAccess-
Palavras-chave: dc.subjectforensic science-
Palavras-chave: dc.subjectmitochondrial DNA-
Palavras-chave: dc.subjectheteroplasmy-
Palavras-chave: dc.subjecthair-
Palavras-chave: dc.subjectblood-
Palavras-chave: dc.subjectHV3-
Título: dc.titleHeteroplasmy in Hair: Study of Mitochondrial DNA Third Hypervariable Region in Hair and Blood Samples-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.