Catecholamine effects on human melanoma cells evoked by α1-adrenoceptors

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorScarparo, Ana Cristina-
Autor(es): dc.creatorSumida, Doris Hissako-
Autor(es): dc.creatorPatrão, Marília T. C. C.-
Autor(es): dc.creatorAvellar, Maria Christina W.-
Autor(es): dc.creatorVisconti, Maria Aparecida-
Autor(es): dc.creatorCastrucci, Ana Maria de Lauro-
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Descrição: dc.descriptionThe biological effects of catecholamines in mammalian pigment cells are poorly understood. Our previous results showed the presence of α1-adrenoceptors in SK-Mel 23 human melanoma cells. The aims of this work were to (1) characterize catecholamine effects on proliferation, tyrosinase activity and expression, (2) identify the α1- adrenoceptor subtypes, and (3) verify whether chronic norepinephrine (NE) treatment modified the types and/or pharmacological characteristics of adrenoceptors present in SK-Mel 23 human melanoma cells. Cells treated with the aradrenergic agonist, phenylephrine (PHE, 10-5 or 10-4 M), for 24-72 h, exhibited decreased cell proliferation and enhanced tyrosinase activity, but unaltered tyrosinase expression as compared with the control. The proliferation and tyrosinase activity responses were inhibited by the α1-adrenergic antagonist prazosin, suggesting they were evoked by α1-adrenoceptors. The presence of actinomycin D, a transcription inhibitor, did not diminish PHE-induced effects. RT-PCR assays, followed by cloning and sequencing, demonstrated the presence of α1A- and α1B-adrenoceptor subtypes. NE-treated cells (24 or 72 h) were used in competition assays, and showed no significant change in the competition curves of α1-adrenoceptors as compared with control curves. Other adrenoceptor subtypes were not identified in these cells, and NE pretreatment did not induce their expression. In conclusion, the activation of SK-Mel 23 human melanoma α1- radrenoceptors elicit biological effects, such as proliferation decrease and tyrosinase activity increase. Desensitization or expression of other adrenoceptor subtypes after chronic NE treatment were not observed.-
Formato: dc.format112-119-
Idioma: dc.languageen-
Relação: dc.relationArchives of Dermatological Research-
Relação: dc.relation2.148-
Relação: dc.relation1,006-
Direitos: dc.rightsclosedAccess-
Palavras-chave: dc.subjectα1-Adrenoceptors-
Palavras-chave: dc.subjectCatecholamines-
Palavras-chave: dc.subjectCell proliferation-
Palavras-chave: dc.subjectSK-Mel 23 melanoma cells-
Palavras-chave: dc.subjectTyrosinase activity-
Palavras-chave: dc.subjectalpha 1 adrenergic receptor-
Palavras-chave: dc.subjectalpha 1 adrenergic receptor blocking agent-
Palavras-chave: dc.subjectalpha 1 adrenergic receptor stimulating agent-
Palavras-chave: dc.subjectascorbic acid-
Palavras-chave: dc.subjectbenoxathian-
Palavras-chave: dc.subjectcatecholamine-
Palavras-chave: dc.subjectdactinomycin-
Palavras-chave: dc.subjectmonophenol monooxygenase-
Palavras-chave: dc.subjectnoradrenalin-
Palavras-chave: dc.subjectphenylephrine-
Palavras-chave: dc.subjectprazosin-
Palavras-chave: dc.subjectadrenergic system-
Palavras-chave: dc.subjectcatecholamine metabolism-
Palavras-chave: dc.subjectcell proliferation-
Palavras-chave: dc.subjectcell strain SK Mel 23-
Palavras-chave: dc.subjectcontrolled study-
Palavras-chave: dc.subjectenzyme activity-
Palavras-chave: dc.subjecthuman-
Palavras-chave: dc.subjecthuman cell-
Palavras-chave: dc.subjectmelanoma cell-
Palavras-chave: dc.subjectpigment cell-
Palavras-chave: dc.subjectpriority journal-
Palavras-chave: dc.subjectreverse transcription polymerase chain reaction-
Palavras-chave: dc.subjectWestern blotting-
Palavras-chave: dc.subjectAdrenergic alpha-Agonists-
Palavras-chave: dc.subjectBinding, Competitive-
Palavras-chave: dc.subjectCell Division-
Palavras-chave: dc.subjectCell Line, Tumor-
Palavras-chave: dc.subjectGene Expression-
Palavras-chave: dc.subjectHumans-
Palavras-chave: dc.subjectMelanoma-
Palavras-chave: dc.subjectMonophenol Monooxygenase-
Palavras-chave: dc.subjectNorepinephrine-
Palavras-chave: dc.subjectPhenylephrine-
Palavras-chave: dc.subjectReceptors, Adrenergic, alpha-1-
Palavras-chave: dc.subjectSkin Neoplasms-
Título: dc.titleCatecholamine effects on human melanoma cells evoked by α1-adrenoceptors-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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