Ultrastructural analysis of bovine oocytes from ovarian follicles with different diameters

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorLisboa, Lvia Aires-
Autor(es): dc.creatorAndrade, Evelyn Rabelo-
Autor(es): dc.creatorEsper, Cesar Roberto-
Autor(es): dc.creatorSeneda, Marcelo Marcondes-
Data de aceite: dc.date.accessioned2021-03-10T19:09:41Z-
Data de disponibilização: dc.date.available2021-03-10T19:09:41Z-
Data de envio: dc.date.issued2014-05-20-
Data de envio: dc.date.issued2014-05-20-
Data de envio: dc.date.issued2011-01-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.5433/1679-0359.2011v32n4p1575-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/42621-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/42621-
Descrição: dc.descriptionIn vitro embryo production is an important technique for facilitating the reproduction of animals with high genetic merit. The greatest challenge for the reproducibility of this technique is the quality of the oocyte that is submitted for in vitro maturation. The aim of this work was to evaluate the ultrastructural characteristics of oocytes from follicles of different diameters using transmission electron microscopy. The animals were divided into 2 groups and were given a single i.m. injection of 250 IU FSH (Pluset, Serono, Italy). To synchronize the follicular growth, all follicles > 2 mm were aspirated during the estrous cycle, which was considered day zero (D0). Group 1 (G1; n = 4) received FSH on day 1 (D1), and the 2- to 5-mm follicles were aspirated on day 2 (D2). The animals in group 2 (G2; n = 5) received FSH on day 2 (D2), and their 10- to 15-mm follicles were aspirated on day 5 (D5). After aspiration, the oocytes from both groups were fixed and prepared for ultrastructural analysis. The oocytes analyzed from both groups had similar ultrastructural characteristics. The presence and distribution of organelles in the cytoplasm of the oocytes did not differ between groups, suggesting that, in relation to the ultrastructural characteristics, oocytes from 2 to 5 mm and 10 to 15 mm follicles are similar.-
Formato: dc.format1575-1582-
Idioma: dc.languageen-
Publicador: dc.publisherUniversidade Estadual de Londrina (UEL)-
Relação: dc.relationSemina: Ciências Agrárias-
Relação: dc.relation0.349-
Direitos: dc.rightsopenAccess-
Palavras-chave: dc.subjectFollicle size-
Palavras-chave: dc.subjectOPU-
Palavras-chave: dc.subjectElectron microscopy-
Título: dc.titleUltrastructural analysis of bovine oocytes from ovarian follicles with different diameters-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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