Xylella fastidiosa gene expression analysis by DNA microarrays

Registro completo de metadados
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorTravensolo, Regiane F.-
Autor(es): dc.creatorCarareto-Alves, Lucia M.-
Autor(es): dc.creatorCosta, Maria V. C. G.-
Autor(es): dc.creatorLopes, Tiago J. S.-
Autor(es): dc.creatorCarrilho, Emanuel-
Autor(es): dc.creatorLemos, Eliana G. M.-
Data de aceite: dc.date.accessioned2021-03-10T16:38:32Z-
Data de disponibilização: dc.date.available2021-03-10T16:38:32Z-
Data de envio: dc.date.issued2014-05-20-
Data de envio: dc.date.issued2014-05-20-
Data de envio: dc.date.issued2009-01-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1590/S1415-47572009005000038-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/4066-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/4066-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionProcesso FAPESP: 00/06289-2-
Descrição: dc.descriptionXylella fastidiosa genome sequencing has generated valuable data by identifying genes acting either on metabolic pathways or in associated pathogenicity and virulence. Based on available information on these genes, new strategies for studying their expression patterns, such as microarray technology, were employed. A total of 2,600 primer pairs were synthesized and then used to generate fragments using the PCR technique. The arrays were hybridized against cDNAs labeled during reverse transcription reactions and which were obtained from bacteria grown under two different conditions (liquid XDM(2) and liquid BCYE). All data were statistically analyzed to verify which genes were differentially expressed. In addition to exploring conditions for X. fastidiosa genome-wide transcriptome analysis, the present work observed the differential expression of several classes of genes (energy, protein, amino acid and nucleotide metabolism, transport, degradation of substances, toxins and hypothetical proteins, among others). The understanding of expressed genes in these two different media will be useful in comprehending the metabolic characteristics of X. fastidiosa, and in evaluating how important certain genes are for the functioning and survival of these bacteria in plants.-
Formato: dc.format340-353-
Idioma: dc.languageen-
Publicador: dc.publisherSoc Brasil Genetica-
Relação: dc.relationGenetics and Molecular Biology-
Relação: dc.relation1.493-
Relação: dc.relation0,638-
Direitos: dc.rightsopenAccess-
Palavras-chave: dc.subjectXylella fastidiosa-
Palavras-chave: dc.subjectDNA microarray-
Palavras-chave: dc.subjectGene expression-
Título: dc.titleXylella fastidiosa gene expression analysis by DNA microarrays-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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