MALDI–TOF bacterial subtyping for rapid detection of biomarkers in Staphylococcus aureus from subclinical bovine mastitis

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorFederal Rural University of Pernambuco-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorMichigan State University-
Autor(es): dc.creatorLopes, Thainara-
Autor(es): dc.creatorFidelis, Carlos E.-
Autor(es): dc.creatorSilva, Amanda T.F.-
Autor(es): dc.creatorMota, Rinaldo A.-
Autor(es): dc.creatorRall, Vera L.M.-
Autor(es): dc.creatordos Santos, Marcos V.-
Autor(es): dc.creatorGonçalves, Juliano L.-
Data de aceite: dc.date.accessioned2025-08-21T18:58:34Z-
Data de disponibilização: dc.date.available2025-08-21T18:58:34Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-10-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1093/jambio/lxad249-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/310007-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/310007-
Descrição: dc.descriptionAims: This study aimed to evaluate matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) bacterial subtyping for the rapid detection of biomarkers in Staphylococcus aureus from subclinical bovine mastitis. Methods and results: A total of 229 S. aureus isolates were obtained from milk samples collected from cows with subclinical mastitis using microbiological culture. Staphylococcus aureus isolates were also submitted to PCR analysis targeting the mecA and mecC genes, which are indicative of methicillin resistance. Confirmation of the species was achieved through MALDI–TOF MS analysis. To analyze antimicrobial resistance patterns, the MALDI BioTyper Compass Explorer and ClinProTools Bruker software were employed, and dendrograms were generated using Bionumerics software. Conclusions: MALDI–TOF MS successfully identified S. aureus at the species level, but no methicillin resistance was observed. Moreover, spectral typing displayed limited similarity when compared to pulsed-field gel electrophoresis (PFGE).-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionSchool of Animal Science and Food Engineering University of São Paulo-
Descrição: dc.descriptionQualileite Department of Animal Nutrition and Production School of Veterinary Medicine and Animal Sciences University of São Paulo-
Descrição: dc.descriptionDepartment of Veterinary Medicine Federal Rural University of Pernambuco-
Descrição: dc.descriptionDepartment of Chemical and Biological Sciences Institute of Biosciences São Paulo State University-
Descrição: dc.descriptionDepartment of Large Animal Clinical Sciences College of Veterinary Medicine Michigan State University-
Descrição: dc.descriptionDepartment of Chemical and Biological Sciences Institute of Biosciences São Paulo State University-
Descrição: dc.descriptionFAPESP: 2013/23613-8-
Descrição: dc.descriptionFAPESP: 2014/17411-6-
Descrição: dc.descriptionFAPESP: 2015/04570-1-
Idioma: dc.languageen-
Relação: dc.relationJournal of Applied Microbiology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectbiomarkers-
Palavras-chave: dc.subjectMALDI–TOF MS-
Palavras-chave: dc.subjectmastitis-
Palavras-chave: dc.subjectPCR-
Palavras-chave: dc.subjectresistance-
Palavras-chave: dc.subjectStaphylococcus aureus-
Título: dc.titleMALDI–TOF bacterial subtyping for rapid detection of biomarkers in Staphylococcus aureus from subclinical bovine mastitis-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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