Satellitome Analysis of Adalia bipunctata (Coleoptera): Revealing Centromeric Turnover and Potential Chromosome Rearrangements in a Comparative Interspecific Study

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversity of Jaén-
Autor(es): dc.contributorAutonomous University of Madrid-
Autor(es): dc.contributorUniversity of the Republic-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorMora, Pablo-
Autor(es): dc.creatorRico-Porras, José M.-
Autor(es): dc.creatorPalomeque, Teresa-
Autor(es): dc.creatorMontiel, Eugenia E.-
Autor(es): dc.creatorPita, Sebastián-
Autor(es): dc.creatorCabral-de-Mello, Diogo C.-
Autor(es): dc.creatorLorite, Pedro-
Data de aceite: dc.date.accessioned2025-08-21T21:42:23Z-
Data de disponibilização: dc.date.available2025-08-21T21:42:23Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-09-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/ijms25179214-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/309383-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/309383-
Descrição: dc.descriptionEukaryotic genomes exhibit a dynamic interplay between single-copy sequences and repetitive DNA elements, with satellite DNA (satDNA) representing a substantial portion, mainly situated at telomeric and centromeric chromosomal regions. We utilized Illumina next-generation sequencing data from Adalia bipunctata to investigate its satellitome. Cytogenetic mapping via fluorescence in situ hybridization was performed for the most abundant satDNA families. In silico localization of satDNAs was carried out using the CHRISMAPP (Chromosome In Silico Mapping) pipeline on the high-fidelity chromosome-level assembly already available for this species, enabling a meticulous characterization and localization of multiple satDNA families. Additionally, we analyzed the conservation of the satellitome at an interspecific scale. Specifically, we employed the CHRISMAPP pipeline to map the satDNAs of A. bipunctata onto the genome of Adalia decempunctata, which has also been sequenced and assembled at the chromosome level. This analysis, along with the creation of a synteny map between the two species, suggests a rapid turnover of centromeric satDNA between these species and the potential occurrence of chromosomal rearrangements, despite the considerable conservation of their satellitomes. Specific satDNA families in the sex chromosomes of both species suggest a role in sex chromosome differentiation. Our interspecific comparative study can provide a significant advance in the understanding of the repeat genome organization and evolution in beetles.-
Descrição: dc.descriptionDepartment of Experimental Biology Genetics Area University of Jaén, Paraje las Lagunillas s/n-
Descrição: dc.descriptionDepartment of Biology Genetics Faculty of Sciences Autonomous University of Madrid-
Descrição: dc.descriptionBiodiversity and Global Change Research Centre (CIBC-UAM) Autonomous University of Madrid-
Descrição: dc.descriptionSection Evolutive Genetics Faculty of Sciences University of the Republic, Iguá 4225-
Descrição: dc.descriptionDepartment of General and Applied Biology Institute of Biosciences/IB UNESP—São Paulo State University, SP-
Descrição: dc.descriptionDepartment of General and Applied Biology Institute of Biosciences/IB UNESP—São Paulo State University, SP-
Idioma: dc.languageen-
Relação: dc.relationInternational Journal of Molecular Sciences-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAdalia bipunctata-
Palavras-chave: dc.subjectAdalia decempunctata-
Palavras-chave: dc.subjectChromosome In Silico Mapping (CHRISMAPP)-
Palavras-chave: dc.subjectCoccinellidae-
Palavras-chave: dc.subjectColeoptera-
Palavras-chave: dc.subjectfluorescence in situ hybridization (FISH)-
Palavras-chave: dc.subjectkaryotype evolution-
Palavras-chave: dc.subjectladybird beetle-
Palavras-chave: dc.subjectRepeatExplorer2-
Palavras-chave: dc.subjectsatellite DNA-
Palavras-chave: dc.subjectsatellitome-
Título: dc.titleSatellitome Analysis of Adalia bipunctata (Coleoptera): Revealing Centromeric Turnover and Potential Chromosome Rearrangements in a Comparative Interspecific Study-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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