Effects of glass–ceramic produced by the sol–gel route in macrophages recruitment and polarization into bone tissue regeneration

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorThe University of Texas Rio Grande Valley – UTRGV-
Autor(es): dc.contributorCentro Universitário Sagrado Coração-
Autor(es): dc.contributorSão Carlos Federal University-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorda Silva, Raquel Barroso Parra-
Autor(es): dc.creatorBiguetti, Claudia Cristina-
Autor(es): dc.creatorMunerato, Marcelo Salles-
Autor(es): dc.creatorSiqueira, Renato Luis-
Autor(es): dc.creatorZanotto, Edgard Dutra-
Autor(es): dc.creatorKudo, Guilherme Halawa Abu-
Autor(es): dc.creatorSimionato, Gustavo Baroni-
Autor(es): dc.creatorBacelar, Ana Carolina Zucon-
Autor(es): dc.creatorOrtiz, Rafael Carneiro-
Autor(es): dc.creatorFerreira-Junior, Joel Santiago-
Autor(es): dc.creatorRangel-Junior, Idelmo Garcia-
Autor(es): dc.creatorMatsumoto, Mariza Akemi-
Data de aceite: dc.date.accessioned2025-08-21T18:21:14Z-
Data de disponibilização: dc.date.available2025-08-21T18:21:14Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1002/jbm.b.35340-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/309378-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/309378-
Descrição: dc.descriptionEffective bone substitute biomaterials remain an important challenge in patients with large bone defects. Glass ceramics produced by different synthesis routes may result in changes in the material physicochemical properties and consequently affect the success or failure of the bone healing response. To investigate the differences in the orchestration of the inflammatory and healing process in bone grafting and repair using different glass–ceramic routes production. Thirty male Wistar rats underwent surgical unilateral parietal defects filled with silicate glass–ceramic produced by distinct routes: BS – particulate glass–ceramic produced via the fusion/solidification route, and BG – particulate glass–ceramic produced via the sol–gel route. After 7, 14, and 21 days from biomaterial grafting, parietal bones were removed to be analyzed under H&E and Massons' Trichome staining, and immunohistochemistry for CD206, iNOS, and TGF-β. Our findings demonstrated that the density of lymphocytes and plasma cells was significantly higher in the BS group at 45, and 7 days compared to the BG group, respectively. Furthermore, a significant increase of foreign body giant cells (FBGCs) in the BG group at day 7, compared to BS was found, demonstrating early efficient recruitment of FBGCs against sol–gel-derived glass–ceramic particulate (BS group). According to macrophage profiles, CD206+ macrophages enhanced at the final periods of both groups, being significantly higher at 45 days of BS compared to the BG group. On the other hand, the density of transformation growth factor beta (TGF-β) positive cells on 21 days were the highest in BG, and the lowest in the BS group, demonstrating a differential synergy among groups. Noteworthy, TGF-β+ cells were significantly higher at 21 days of BG compared to the BS group. Glass–ceramic biomaterials can act differently in the biological process of bone remodeling due to their route production, being the sol–gel route more efficient to activate M2 macrophages and specific FBGCs compared to the traditional route. Altogether, these features lead to a better understanding of the effectiveness of inflammatory response for biomaterial degradation and provide new insights for further preclinical and clinical studies involved in bone healing.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Basic Sciences São Paulo State University (Unesp) School of Dentistry-
Descrição: dc.descriptionRegenerative Medicine Laboratory School of Podiatric Medicine The University of Texas Rio Grande Valley – UTRGV-
Descrição: dc.descriptionDepartment of Health Sciences Centro Universitário Sagrado Coração-
Descrição: dc.descriptionDepartment of Material Engineering São Carlos Federal University-
Descrição: dc.descriptionHospital for Rehabilitation of Craniofacial Anomalies University of São Paulo-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery São Paulo State University (Unesp) School of Dentistry-
Descrição: dc.descriptionDepartment of Basic Sciences São Paulo State University (Unesp) School of Dentistry-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery São Paulo State University (Unesp) School of Dentistry-
Descrição: dc.descriptionFAPESP: 2016/03762-7-
Idioma: dc.languageen-
Relação: dc.relationJournal of Biomedical Materials Research - Part B Applied Biomaterials-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectbiomaterial-
Palavras-chave: dc.subjectbone repair-
Palavras-chave: dc.subjectglass–ceramic-
Palavras-chave: dc.subjectinflammation-
Palavras-chave: dc.subjectM2 macrophages-
Palavras-chave: dc.subjectsol–gel route-
Título: dc.titleEffects of glass–ceramic produced by the sol–gel route in macrophages recruitment and polarization into bone tissue regeneration-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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