Combined catalytic strategies applied to in-office tooth bleaching: whitening efficacy, cytotoxicity, and gene expression of human dental pulp cells in a 3D culture model

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorde Oliveira Ribeiro, Rafael Antonio-
Autor(es): dc.creatorPeruchi, Victória-
Autor(es): dc.creatorSores, Igor Paulino Mendes-
Autor(es): dc.creatorMon, Filipe Koon Wu-
Autor(es): dc.creatorSoares, Diana Gabriela-
Autor(es): dc.creatorHebling, Josimeri-
Autor(es): dc.creatorde Souza Costa, Carlos Alberto-
Data de aceite: dc.date.accessioned2025-08-21T21:44:17Z-
Data de disponibilização: dc.date.available2025-08-21T21:44:17Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s00784-024-06069-4-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/309331-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/309331-
Descrição: dc.descriptionObjectives: To evaluate the feasibility of using a 3D model with human dental pulp cells (HDPCs) to compare bleaching therapies and assess whether coating enamel with a nanofiber scaffold (NS) and polymeric catalyst primer (PCP), combined with violet LED (LEDv) irradiation, enhances bleaching efficacy (BE) and reduces cytotoxicity (CT). Materials and methods: After using NS + PCP to cover enamel of enamel/dentin discs adapted to artificial pulp chambers containing 3D culture with HDPCs, a bleaching gel with 35%H2O2 was applied and then irradiated with LEDv. The following groups were established (n = 8): NC - no treatment; PC– 35%H2O2 for 45 min, and EXP: NS + PCP + 35%H2O2 + LEDv for 15 min. The study evaluated BE (ΔE00 and ΔWID), CT (alamarBlue), and HDPCs gene modulation (TNF, IL1B, PTGS2, IL8, IL6, PPRAG, HMOX1, DSPP, DMP1, SPP1, BGLAP, and ALPL; RTqPCR). Results: BE showed no significant difference between PC and EXP (p > 0.05). EXP had lower oxidative stress, higher cell viability, reduced inflammatory marker expression, and increased mineralization marker expression compared to PC (p < 0.05). Conclusion: The 3D model using HDPCs effectively compared bleaching protocols. Coating enamel with NS + PCP and applying violet LED (LEDv) reduced bleaching time from 45 to 15 min while lowering cytotoxicity compared to conventional in-office treatments. Clinical relevance: This study shows that a 3D model with human dental pulp cells (HDPCs) effectively compares tooth bleaching protocols. The combination of a nanofiber scaffold with violet LED (LEDv) reduces bleaching time, as well as minimizes cytotoxicity and inflammation, offering a safer alternative to conventional treatments.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Operative Dentistry Endodontics and Dental Materials School of Dentistry University of São Paulo (USP)-
Descrição: dc.descriptionDepartment of Morphology Genetics Orthodontics and Pediatric Dentistry School of Dentistry São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Physiology and Pathology School of Dentistry São Paulo State University (UNESP), Rua Humaitá-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Morphology Genetics Orthodontics and Pediatric Dentistry School of Dentistry São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Physiology and Pathology School of Dentistry São Paulo State University (UNESP), Rua Humaitá-
Descrição: dc.descriptionFAPESP: 2021/01184-4-
Descrição: dc.descriptionFAPESP: 2023/02780-5-
Descrição: dc.descriptionCNPq: 302047/2019-0-
Idioma: dc.languageen-
Relação: dc.relationClinical Oral Investigations-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subject3D culture-
Palavras-chave: dc.subjectCatalysis-
Palavras-chave: dc.subjectDental pulp-
Palavras-chave: dc.subjectGene expression-
Palavras-chave: dc.subjectTooth bleaching-
Título: dc.titleCombined catalytic strategies applied to in-office tooth bleaching: whitening efficacy, cytotoxicity, and gene expression of human dental pulp cells in a 3D culture model-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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