Comparative analysis of MALDI-TOF MS and RAPD for grouping Streptococcus agalactiae isolated from subclinical mastitis isolates

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Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorPereira, Ligia Beatriz Rizzanti-
Autor(es): dc.creatorGarcia, Breno Luis Nery-
Autor(es): dc.creatorFidelis, Carlos Eduardo-
Autor(es): dc.creatorBarbosa, Kristian da Silva-
Autor(es): dc.creatorDantas, Stéfani Thais Alves-
Autor(es): dc.creatorRall, Vera Lúcia Mores-
Autor(es): dc.creatordos Santos, Marcos Veiga-
Data de aceite: dc.date.accessioned2025-08-21T19:56:26Z-
Data de disponibilização: dc.date.available2025-08-21T19:56:26Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2025-07-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.micpath.2025.107538-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/309263-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/309263-
Descrição: dc.descriptionStreptococcus agalactiae is a primary pathogen associated with subclinical mastitis in dairy herds, requiring accurate identification and characterization for effective management due to its highly contagious nature. This study evaluated the use of Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) for grouping S. agalactiae isolates from subclinical mastitis in cattle and buffaloes associating the antimicrobial susceptibility profiles of the isolates with the grouping results. A total of 198 milk samples were collected from three farms (Farm A: 67 cow isolates, Farm B: 101 cow isolates, Farm C: 30 buffalo isolates). Antimicrobial susceptibility testing using the Minimum Inhibitory Concentration (MIC) method was performed for 10 antimicrobials. High sensitivity (>90 %) was observed for ceftiofur, penicillin, oxacillin, amoxicillin, cefquinome, gentamicin, and cefoxitin, while low sensitivity (<15 %) was detected for enrofloxacin and cephalexin. Clustering was conducted using Random Amplified Polymorphic DNA (RAPD) and grouped by MALDI-TOF MS. RAPD identified 33 clusters at an 80 % similarity breakpoint, while MALDI-TOF MS identified 8 distinct groups. MALDI-TOF MS successfully grouped all isolates, whereas RAPD clustered only 100. Both methods grouped isolates from the same herd with similar susceptibility profiles. These findings highlight the use of MALDI-TOF MS for rapid grouping pathogens but emphasize differences when compared to RAPD.-
Descrição: dc.descriptionDepartment of Animal Nutrition and Production School of Veterinary Medicine and Animal Sciences University of São Paulo, SP-
Descrição: dc.descriptionDepartment of Chemical and Biological Sciences Institute of Biosciences São Paulo State University, SP-
Descrição: dc.descriptionDepartment of Chemical and Biological Sciences Institute of Biosciences São Paulo State University, SP-
Idioma: dc.languageen-
Relação: dc.relationMicrobial Pathogenesis-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAntimicrobial resistance-
Palavras-chave: dc.subjectBovine mastitis-
Palavras-chave: dc.subjectMALDI-TOF MS-
Palavras-chave: dc.subjectRAPD-
Palavras-chave: dc.subjectStreptococcus agalactiae-
Título: dc.titleComparative analysis of MALDI-TOF MS and RAPD for grouping Streptococcus agalactiae isolated from subclinical mastitis isolates-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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