Peptidomics of Zebrafish Brain in a 6-OHDA-Induced Neurodegeneration Model

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorFiametti, Louise O.-
Autor(es): dc.creatorde Mello, Felipe Ricardo-
Autor(es): dc.creatorde Castro, Leandro M.-
Data de aceite: dc.date.accessioned2025-08-21T23:01:58Z-
Data de disponibilização: dc.date.available2025-08-21T23:01:58Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/978-1-0716-3646-6_16-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/309206-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/309206-
Descrição: dc.descriptionBioactive peptides such as neuropeptides and peptide hormones are largely understood in their involvement in a variety of physiologic systems. In addition to the neuropeptides produced and processed by the classic secretory pathway, intracellular peptides (InPeps) have shown biological activity in studies involving different organisms. A model that has become attractive in many research fields is the zebrafish (Danio rerio), which has allowed correlating behavioral responses or physiological processes with underlying molecular pathways or signaling cascades, improving the understanding of homeostasis mechanisms of the central nervous system, as well as pathological processes such as neurodegenerative diseases. Here, we provide a detailed description of the protocol of treatment with 6-OHDA, which mimics some features of Parkinson’s Disease, as well as the validation of the treatment by evaluation of the locomotor activity and the protocol of peptide extraction followed by isotopic labeling to peptide relative quantitation by mass spectrometry.-
Descrição: dc.descriptionSao Paulo State University (UNESP) Bioscience Institute-
Descrição: dc.descriptionBiodiversity of Coastal Environments Postgraduate Program Sao Paulo State University (UNESP) Bioscience Institute-
Descrição: dc.descriptionSao Paulo State University (UNESP) Bioscience Institute-
Descrição: dc.descriptionBiodiversity of Coastal Environments Postgraduate Program Sao Paulo State University (UNESP) Bioscience Institute-
Formato: dc.format307-317-
Idioma: dc.languageen-
Relação: dc.relationMethods in Molecular Biology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectDanio rerio-
Palavras-chave: dc.subjectIntracellular peptides-
Palavras-chave: dc.subjectMass spectrometry-
Palavras-chave: dc.subjectNeuropeptide-
Palavras-chave: dc.subjectParkinson’s Disease-
Palavras-chave: dc.subjectPeptidomics-
Palavras-chave: dc.subjectZebrafish-
Título: dc.titlePeptidomics of Zebrafish Brain in a 6-OHDA-Induced Neurodegeneration Model-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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