Artemisia vulgaris Induces Tumor-Selective Ferroptosis and Necroptosis via Lysosomal Ca2+ Signaling

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Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorAnhembi Morumbi University-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorZamarioli, Lucas dos Santos-
Autor(es): dc.creatorSantos, Michele Rosana Maia-
Autor(es): dc.creatorErustes, Adolfo Garcia-
Autor(es): dc.creatorMeccatti, Vanessa Marques-
Autor(es): dc.creatorPereira, Thaís Cristine-
Autor(es): dc.creatorSmaili, Soraya S.-
Autor(es): dc.creatorMarcucci, Maria Cristina-
Autor(es): dc.creatorOliveira, Carlos Rocha-
Autor(es): dc.creatorPereira, Gustavo J. S.-
Autor(es): dc.creatorBincoletto, Claudia-
Data de aceite: dc.date.accessioned2025-08-21T21:18:31Z-
Data de disponibilização: dc.date.available2025-08-21T21:18:31Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-06-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s11655-023-3712-2-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/309078-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/309078-
Descrição: dc.descriptionObjective: To evaluate the chemical composition and effects of Artemisia vulgaris (AV) hydroalcoholic extract (HEAV) on breast cancer cells (MCF-7 and SKBR-3), chronic myeloid leukemia (K562) and NIH/3T3 fibroblasts. Methods: Phytochemical analysis of HEAV was done by high-performance liquid chromatography-mass (HPLC) spectrometry. Viability and cell death studies were performed using trypan blue and Annexin/FITC-7AAD, respectively. Ferrostatin-1 (Fer-1) and necrostatin-1 (Nec-1) were used to assess the mode of HEAV-induced cell death and acetoxymethylester (BAPTA-AM) was used to verify the involvement of cytosolic calcium in this event. Cytosolic calcium measurements were made using Fura-2-AM. Results: HEAV decreased the viability of MCF-7, SKBR-3 and K562 cells (P<0.05). The viability of HEAV-treated K562 cells was reduced compared to HEAV-exposed fibroblasts (P<0.05). Treatment of K562 cells with HEAV induced cell death primarily by late apoptosis and necrosis in assays using annexin V-FITC/7-AAD (P<0.05). The use of Nec-1 and Fer-1 increased the viability of K562 cells treated with HEAV relative to cells exposed to HEAV alone (P<0.01). HEAV-induced Ca2+ release mainly from lysosomes in K562 cells (P<0.01). Furthermore, BAPTA-AM, an intracellular Ca2+ chelator, decreased the number of non-viable cells treated with HEAV (P<0.05). Conclusions: HEAV is cytotoxic and activates several modalities of cell death, which are partially dependent on lysosomal release of Ca2+. These effects may be related to artemisinin and caffeoylquinic acids, the main compounds identified in HEAV.-
Descrição: dc.descriptionDepartment of Pharmacology Paulista School of Medicine Federal University of São Paulo, SP-
Descrição: dc.descriptionResearch Group on Phytocomplexes and Cell Signaling School of Health Sciences Anhembi Morumbi University, SP-
Descrição: dc.descriptionDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (ICT-UNESP), SP-
Descrição: dc.descriptionDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (ICT-UNESP), SP-
Formato: dc.format525-533-
Idioma: dc.languageen-
Relação: dc.relationChinese Journal of Integrative Medicine-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectArtemisia vulgaris-
Palavras-chave: dc.subjectCa2+ signaling-
Palavras-chave: dc.subjectferroptosis-
Palavras-chave: dc.subjectlate apoptosis-
Palavras-chave: dc.subjectnecroptosis-
Título: dc.titleArtemisia vulgaris Induces Tumor-Selective Ferroptosis and Necroptosis via Lysosomal Ca2+ Signaling-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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