Biocompatibility and inflammatory response of silver tungstate, silver molybdate, and silver vanadate microcrystals

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversity Jaume I (UJI)-
Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.creatorPimentel, Bruna Natália Alves da Silva-
Autor(es): dc.creatorDe Annunzio, Sarah Raquel-
Autor(es): dc.creatorAssis, Marcelo-
Autor(es): dc.creatorBarbugli, Paula Aboud-
Autor(es): dc.creatorLongo, Elson-
Autor(es): dc.creatorVergani, Carlos Eduardo-
Data de aceite: dc.date.accessioned2025-08-21T17:48:49Z-
Data de disponibilização: dc.date.available2025-08-21T17:48:49Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2022-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3389/fbioe.2023.1215438-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/309014-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/309014-
Descrição: dc.descriptionSilver tungstate (α-Ag2WO4), silver molybdate (β-Ag2MoO4), and silver vanadate (α-AgVO3) microcrystals have shown interesting antimicrobial properties. However, their biocompatibility is not yet fully understood. Cytotoxicity and the inflammatory response of silver-containing microcrystals were analyzed in THP-1 and THP-1 differentiated as macrophage-like cells, with the alamarBlue™ assay, flow cytometry, confocal microscopy, and ELISA. The present investigation also evaluated redox signaling and the production of cytokines (TNFα, IL-1β, IL-6, and IL-8) and matrix metalloproteinases (MMP-8 and -9). The results showed that α-AgVO3 (3.9 μg/mL) did not affect cell viability (p > 0.05). α-Ag2WO4 (7.81 μg/mL), β-Ag2MoO4 (15.62 μg/mL), and α-AgVO3 (15.62 μg/mL) slightly decreased cell viability (p ≤ 0.003). All silver-containing microcrystals induced the production of O2− and this effect was mitigated by Reactive Oxygen Species (ROS) scavenger and N-acetylcysteine (NAC). TNFα, IL-6 and IL-1β were not detected in THP-1 cells, while their production was either lower (p ≤ 0.0321) or similar to the control group (p ≥ 0.1048) for macrophage-like cells. The production of IL-8 by both cellular phenotypes was similar to the control group (p ≥ 0.3570). The release of MMP-8 was not detected in any condition in THP-1 cells. Although MMP-9 was released by THP-1 cells exposed to α-AgVO3 (3.9 μg/mL), no significant difference was found with control (p = 0.7). Regarding macrophage-like cells, the release of MMP-8 and -9 decreased in the presence of all microcrystals (p ≤ 0.010). Overall, the present work shows a promising biocompatibility profile of, α-Ag2WO4, β-Ag2MoO4, and α-AgVO3 microcrystals.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionSchool of Dentistry São Paulo State University (Unesp)-
Descrição: dc.descriptionDepartament of Physical and Analytical Chemistry University Jaume I (UJI)-
Descrição: dc.descriptionCDMF Federal University of São Carlos (UFSCar)-
Descrição: dc.descriptionSchool of Dentistry São Paulo State University (Unesp)-
Descrição: dc.descriptionFAPESP: 2013/07296-2 2018/01677-8 2022/08487-5-
Idioma: dc.languageen-
Relação: dc.relationFrontiers in Bioengineering and Biotechnology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectcytokines-
Palavras-chave: dc.subjectmacrophages-
Palavras-chave: dc.subjectmatrix metalloproteinases, reactive oxygen species-
Palavras-chave: dc.subjectmonocytes-
Palavras-chave: dc.subjectsilver-based metal oxides-
Título: dc.titleBiocompatibility and inflammatory response of silver tungstate, silver molybdate, and silver vanadate microcrystals-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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