Acquired pellicle and biofilm engineering with CaneCPI-5: Insights from proteomic and microbiomics analysis

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Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorSaarland University-
Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorAraujo, Tamara Teodoro-
Autor(es): dc.creatorDionizio, Aline-
Autor(es): dc.creatorCarvalho, Thamyris de Souza-
Autor(es): dc.creatorDebortolli, Ana Luiza Bogaz-
Autor(es): dc.creatorVertuan, Mariele-
Autor(es): dc.creatorde Souza, Beatriz Martines-
Autor(es): dc.creatorCamara, João Victor Frazão-
Autor(es): dc.creatorHenrique-Silva, Flavio-
Autor(es): dc.creatorChiaratti, Marcos-
Autor(es): dc.creatorSantos, Angélica-
Autor(es): dc.creatorAlves, Lindomar-
Autor(es): dc.creatorFerro, Milene-
Autor(es): dc.creatorMagalhães, Ana Carolina-
Autor(es): dc.creatorBuzalaf, Marília Afonso Rabelo-
Data de aceite: dc.date.accessioned2025-08-21T18:14:40Z-
Data de disponibilização: dc.date.available2025-08-21T18:14:40Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-10-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.archoralbio.2024.106025-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/308719-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/308719-
Descrição: dc.descriptionObjective: In this in vivo proof-of-concept study, acquired pellicle engineering was implemented to promote alterations in the protein composition of the acquired enamel pellicle (AEP) and the bacterial composition of the dental biofilm after treatment with Sugarcane cystatin (CaneCPI-5). Design: After prophylaxis, 10 volunteers rinsed (10 mL, 1 min) with the following solutions: 1) deionized water (H2O- negative control or 2) 0.1 mg/mL CaneCPI-5. The AEP and biofilm were formed along 2 or 3 h, respectively. The AEP was collected with electrode filter papers soaked in 3 % citric acid. After protein extraction, samples were analyzed by quantitative shotgun label-free proteomics. The biofilm microbiome was collected with a dental curette. The DNA was extracted, amplified, and analyzed by 16S-rRNA Next Generation Sequencing (NGS). Results: Treatment with CaneCPI-5 increased several proteins with antimicrobial, acid-resistance, affinity for hydroxyapatite, structural and calcium binding properties, such as Cysteine-rich-3 (6-fold-p = 0.03), Cystatin-B (5.5-fold-p < 0.01), Neutrophil-defensin 1 (4.7-fold-p < 0.01), Mucin (3.9-fold-p < 0.01), Immunoglobulin-heavy-constant (3.8-fold-p < 0.01) and Lactotransferrin (2.8-fold-p < 0.01). Microbiome revealed that several commensal bacteria had their abundance increased after rinsing with CaneCPI-5, such as Corynebacterium and Neisseria, while Streptococcus and Prevotella nigrescens were decreased. The results indicate the efficiency of CaneCPI-5 in promoting beneficial changes in the AEP and biofilm, making this phytocystatin a potential target for incorporation into dental products. Conclusion: Cane demonstrated the capability to alter the protein composition of the acquired enamel pellicle (AEP) and the initial colonizers of the biofilm, enhancing the presence of proteins and bacteria crucial for dental protection.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Biological Sciences Bauru School of Dentistry University of São Paulo-
Descrição: dc.descriptionClinic of Operative Dentistry Periodontology and Preventive Dentistry University Hospital Saarland University, Building 73, Saarland-
Descrição: dc.descriptionDepartamento de Genética e Evolução Universidade Federal de São Carlos-
Descrição: dc.descriptionDepartment of General and Applied Biology Paulista State University (UNESP)-
Descrição: dc.descriptionDepartment of General and Applied Biology Paulista State University (UNESP)-
Descrição: dc.descriptionFAPESP: 2019/08032–5-
Descrição: dc.descriptionFAPESP: 2019/26070–1-
Idioma: dc.languageen-
Relação: dc.relationArchives of Oral Biology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectDemineralization-
Palavras-chave: dc.subjectDental caries-
Palavras-chave: dc.subjectMicrobiome-
Palavras-chave: dc.subjectProteome-
Título: dc.titleAcquired pellicle and biofilm engineering with CaneCPI-5: Insights from proteomic and microbiomics analysis-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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