Integrative taxonomy of Metastrongylus spp. in wild boars from Brazil

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorOliveira, Wilson Junior-
Autor(es): dc.creatorPerin, Patricia Parreira-
Autor(es): dc.creatorArias Pacheco, Carmen Andrea-
Autor(es): dc.creatorMendonça, Talita Oliveira-
Autor(es): dc.creatorde Souza Pollo, Andressa-
Autor(es): dc.creatordo Amaral, Renan Bressianini-
Autor(es): dc.creatorde Oliveira Zolla, Natália-
Autor(es): dc.creatorde Oliveira Andrade, Lívia-
Autor(es): dc.creatorGomes, Jonathan Silvestre-
Autor(es): dc.creatordos Santos, Vitória Maximiana Soares-
Autor(es): dc.creatorde Moraes Ferreira, Adrian Felipe-
Autor(es): dc.creatorLux Hoppe, Estevam Guilherme-
Data de aceite: dc.date.accessioned2025-08-21T15:26:10Z-
Data de disponibilização: dc.date.available2025-08-21T15:26:10Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1186/s13071-023-06047-x-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/308604-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/308604-
Descrição: dc.descriptionBackground: Wild boars (Sus scrofa) may cause substantial damage to crops and can spread zoonotic parasites to domestic animals, posing a risk to health and animal production. Metastrongylus spp. can negatively affect the wild boar population, increasing piglet mortality. In addition to that, studies with Metastrongylus genetic characterization are still scarce in Brazil. The present study aims to characterize Metastrongylus spp. from wild boars hunted in the states of São Paulo, Paraná, and Rio Grande do Sul, Brazil, using traditional morphological description and DNA sequences in an integrative taxonomic approach. Methods: After nematode collection from 58 wild boars, the parasites were morphologically identified and genetically characterized by the amplification of 18S ribosomal DNA (rDNA), 28S rDNA, internal transcribed spacer (ITS) region, and cox-1 mitochondrial DNA (mtDNA). Descriptors of infection were determined and Pearson's Chi-square test was applied to compare the prevalence of infections among the identified parasite species, host age group (juveniles and adults), and sex. The Mann–Whitney U test was performed to compare the mean intensity between the age groups and sex. Results: Metastrongylus salmi, Metastrongylus apri, and Metastrongylus pudendotectus were identified in 77.6% (45/58) of the necropsied wild boars. Metastrongylus salmi was the most prevalent and abundant species (70.7%, 11.1), followed by M. pudendotectus (18.9%, 4.3) and M. apri (17.2%, 2.2). Metastrongylus pudendotectus showed the highest mean intensity and range (25.2, 1–93), followed by M. salmi (15.7, 1–58) and M. apri (12.6, 3–27). We found a significantly higher prevalence of Metastrongylus spp. and M. salmi in adult wild boars, probably associated with a more prolonged time of exposure to intermediate host species. The phylogenetic analysis revealed that ITS2 region and cox-1 mtDNA are the most suitable genetic markers for Metastrongylus species characterization. Genetic variability between M. apri and M. salmi isolates was verified. Conclusions: We expand the knowledge about the Metastrongylus community in the non-captive wild boar population from Brazil as well as the importance of this exotic species in the maintenance of Metastrongylus spp. in its areas of occurrence. The novel genetic sequences obtained may help further studies to understand the genetic diversity in other nematode populations from Brazil and other countries. Graphical Abstract: [Figure not available: see fulltext.]-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionInstituto Federal de Alagoas, Ministério da Educação-
Descrição: dc.descriptionMinistério da Educação-
Descrição: dc.descriptionSão Paulo State University, São Paulo-
Descrição: dc.descriptionUniversity of São Paulo, São Paulo-
Descrição: dc.descriptionSão Paulo State University, São Paulo-
Descrição: dc.descriptionCNPq: #311063/2022-5-
Descrição: dc.descriptionCNPq: #407965/2021-1-
Descrição: dc.descriptionFAPESP: 2022/12529-5-
Descrição: dc.descriptionFAPESP: 2022/13548-3-
Descrição: dc.descriptionCAPES: Finance Code 001-
Descrição: dc.descriptionInstituto Federal de Alagoas, Ministério da Educação: MEC/SUS-
Descrição: dc.descriptionMinistério da Educação: MEC/SUS-
Idioma: dc.languageen-
Relação: dc.relationParasites and Vectors-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectInvasive species-
Palavras-chave: dc.subjectLungworms-
Palavras-chave: dc.subjectMetastrongylus-
Palavras-chave: dc.subjectMorphology-
Palavras-chave: dc.subjectNeotropical region-
Palavras-chave: dc.subjectPhylogeny-
Título: dc.titleIntegrative taxonomy of Metastrongylus spp. in wild boars from Brazil-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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