Central obesity is detrimental to anti-inflammatory, phenotype, and exhaustion markers in mononuclear cells - A cross-sectional study

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorRoyal Prince Alfred Hospital-
Autor(es): dc.contributorUniversidade Federal de Ciências da Saúde de Porto Alegre-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorJustus Liebig University Giessen-
Autor(es): dc.contributorUniversity of Coimbra-
Autor(es): dc.creatorOlean-Oliveira, Tiago-
Autor(es): dc.creatorPadilha, Camila S.-
Autor(es): dc.creatorFigueiredo, Caique-
Autor(es): dc.creatorDorneles, Gilson Pires-
Autor(es): dc.creatorMarmett, Bruna-
Autor(es): dc.creatorPeres, Alessandra-
Autor(es): dc.creatorRomão, Pedro-
Autor(es): dc.creatorAbílio de Souza Teixeira, Alexandre-
Autor(es): dc.creatorJabur Ribeiro, José Procópio-
Autor(es): dc.creatorDos Santos, Vanessa Ribeiro-
Autor(es): dc.creatorOlean-Oliveira, André-
Autor(es): dc.creatorTeixeira, Marcos F.S.-
Autor(es): dc.creatorSeraphim, Patrícia M.-
Autor(es): dc.creatorKrüger, Karsten-
Autor(es): dc.creatorRosa-Neto, José Cesar-
Autor(es): dc.creatorLira, Fábio Santos-
Data de aceite: dc.date.accessioned2025-08-21T17:49:55Z-
Data de disponibilização: dc.date.available2025-08-21T17:49:55Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.clnesp.2023.10.035-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/308539-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/308539-
Descrição: dc.descriptionObjective: To investigate the role of central obesity on immunometabolic response in peripheral blood mononuclear cells (PBMCs) from normal weight and overweight/obese young men. Methods: Eighteen individuals were classified as normal weight (NW; n = 9 - age: 25 ± 5 and BMI: 21.4 ± 1.7) and overweight/obese (OW; n = 9 - age: 29 ± 7 and BMI: 29.2 ± 2.7). The body composition was evaluated by dual-energy x-ray absorptiometry (DXA), waist circumference, and visceral and subcutaneous fat depots by ultrasound. Physical activity levels, metabolic parameters, immune phenotypic characterization, cytokine production by lipopolysaccharide (LPS) -stimulated whole blood cells and LPS or phorbol 12-myristate 13-acetate (PMA)-stimulated PBMC, and mitochondrial respiration in PBMCs were evaluated. Expression of AMP-activated protein kinase (AMPK), peroxisome proliferator-activated receptor gamma (PPAR-γ), nuclear factor-kappa B (NF-κB), toll-like receptor 4 (TLR-4), hypoxia-inducible factor-1 alpha (HIF-1α), and adrenergic receptor beta 1 and 2 (AR-β1 and β2) genes were evaluated in cultured PBMC using quantitative real-time polymerase chain reaction (qRT-PCR). Results: Individuals with overweight/obese (OW) presented higher glucose (P = 0.009) and leptin (P = 0.010) than individuals with normal weight (NW). PBMCs of OW under stimulation with LPS presented a lower production of interleukin-10 (IL-10) (P = 0.011) and macrophage inflammatory protein-1alpha (MIP-1α) (P = 0.048) than NW. Mitochondrial respiration rates were not different between NW and OW subjects. Cultured PBMCs in LPS-stimulated condition indicated higher gene expression of AR-β2 in OW, while PMA-stimulated PBMCs presented lower expression of AMPK (P = 0.002) and higher expression of NF-κB (P=<0.0001) than NW. OW presented higher numbers of CD3+CD4+ T cells (P = 0.009) and higher expression of programmed cell death protein 1 (PD-1) in CD8+ T cells (P = 0.001) than NW. Conclusion: Central obesity promoted reductions in interleukin 10 production response and increase in AR-β2 expressions in mitogen-stimulated PBMCs. Furthermore, central obesity altered the phenotype of PBMCs, also increasing the expression of PD-1 exhaustion markers in young adults.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionExercise and Immunometabolism Research Group Post-Graduation Program in Movement Sciences Department of Physical Education São Paulo State University (UNESP), SP-
Descrição: dc.descriptionBiology of Ageing Laboratory Centenary Institute of Cancer Medicine and Cell Biology Royal Prince Alfred Hospital, Missenden Rd-
Descrição: dc.descriptionCellular and Molecular Immunology Lab. Universidade Federal de Ciências da Saúde de Porto Alegre, RS-
Descrição: dc.descriptionImmunometabolism Research Group Department of Cell Biology and Development Institute of Biomedical Science University of São Paulo-
Descrição: dc.descriptionDepartment of Chemistry and Biochemistry School of Science and Technology Sao Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Physiotherapy School of Science and Technology Sao Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Exercise Physiology and Sports Therapy Institute of Sports Science Justus Liebig University Giessen-
Descrição: dc.descriptionCentro de Investigação em Desporto e Atividade Física University of Coimbra-
Descrição: dc.descriptionExercise and Immunometabolism Research Group Post-Graduation Program in Movement Sciences Department of Physical Education São Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Chemistry and Biochemistry School of Science and Technology Sao Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Physiotherapy School of Science and Technology Sao Paulo State University (UNESP)-
Formato: dc.format397-408-
Idioma: dc.languageen-
Relação: dc.relationClinical Nutrition ESPEN-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBody fat-
Palavras-chave: dc.subjectImmune system-
Palavras-chave: dc.subjectImmunometabolism-
Palavras-chave: dc.subjectPhysical fitness-
Título: dc.titleCentral obesity is detrimental to anti-inflammatory, phenotype, and exhaustion markers in mononuclear cells - A cross-sectional study-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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