Influence of different growth conditions on the composition and acidogenicity of saliva-derived microcosm biofilm and their effects on enamel demineralization

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorSampaio, Caio-
Autor(es): dc.creatorCusicanqui Méndez, Daniela Alejandra-
Autor(es): dc.creatorBuzalaf, Marília Afonso Rabelo-
Autor(es): dc.creatorPessan, Juliano Pelim-
Autor(es): dc.creatorCruvinel, Thiago-
Data de aceite: dc.date.accessioned2025-08-21T22:26:26Z-
Data de disponibilização: dc.date.available2025-08-21T22:26:26Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1080/08927014.2024.2410781-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/308454-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/308454-
Descrição: dc.descriptionThis study compared the influence of growth conditions on the composition and acidogenicity of saliva-derived microcosm biofilms and enamel demineralization. Biofilms grown in sucrose-supplemented modified McBain medium, containing 25/50 mmol/L PIPES (buffer), under anaerobiosis/microaerophilia, for 3 and 7 days were evaluated for their acidogenicity, microbial composition, matrix, and enamel mineral content. The viability of total lactobacilli was higher in the group containing 25 mmol/L PIPES grown under anaerobiosis, which also showed lower pH values. The viability of total streptococci and total microorganisms was significantly higher at 7 days in the groups with 50 mmol/L PIPES than at 3 days, regardless of the incubation atmosphere. No significant differences were observed in lactic acid, calcium, superficial hardness loss, or lesion depth. In conclusion, the incubation atmosphere, buffer content in the growth media, and duration of biofilm formation displayed species-varied influence on microcosm biofilms, without causing significant changes in acid metabolism or enamel demineralization.-
Descrição: dc.descriptionDepartment of Preventive and Restorative Dentistry São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Pediatric Dentistry Orthodontics and Public Health Bauru School of Dentistry University of São Paulo-
Descrição: dc.descriptionDepartment of Biological Sciences Bauru School of Dentistry University of São Paulo-
Descrição: dc.descriptionDepartment of Preventive and Restorative Dentistry São Paulo State University (UNESP)-
Formato: dc.format743-753-
Idioma: dc.languageen-
Relação: dc.relationBiofouling-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBiofilm-
Palavras-chave: dc.subjectdental caries-
Palavras-chave: dc.subjectexperimental model-
Palavras-chave: dc.subjectin vitro study-
Palavras-chave: dc.subjectsaliva-
Título: dc.titleInfluence of different growth conditions on the composition and acidogenicity of saliva-derived microcosm biofilm and their effects on enamel demineralization-
Tipo de arquivo: dc.typelivro digital-
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