Interaction of Graphene Oxide Particles and Dendrimers with Human Breast Cancer Cells by Real-Time Microscopy

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorButantan Institute-
Autor(es): dc.contributorFederal University of ABC-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorRibeiro, Beatriz Fumelli Monti-
Autor(es): dc.creatorChaves, Julyane Batista-
Autor(es): dc.creatorDe Souza, Marcelo Medina-
Autor(es): dc.creatorKeppler, Artur Franz-
Autor(es): dc.creatorDo Carmo, Devaney Ribeiro-
Autor(es): dc.creatorMachado-Santelli, Gláucia M.-
Data de aceite: dc.date.accessioned2025-08-21T22:08:01Z-
Data de disponibilização: dc.date.available2025-08-21T22:08:01Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/pharmaceutics15122655-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/308415-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/308415-
Descrição: dc.descriptionGraphene oxide (GOX) has become attractive due to its unique physicochemical properties. This nanomaterial can associate with other dendrimers, making them more soluble and allowing better interaction with biomacromolecules. The present study aimed to investigate, by real-time microscopy, the behavior of human breast cancer cells exposed to particles of materials based on graphene oxide. The MCF-7 cell line was exposed to GOX, GOX associated with Polypropylenimine hexadecaamine Dendrimer, Generation 3.0—DAB-AM-16 (GOXD) and GOX associated with polypropyleneimine—PAMAM (GOXP) in the presence or absence of fetal bovine serum (FBS). GOX, GOXD and GOXP were taken up by the cells in clusters and then the clusters were fragmented into smaller ones inside the cells. Real-time microscopy showed that the presence of FBS in the culture medium could allow a more efficient internalization of graphene materials. After internalizing the materials, cells can redistribute the clumps to their daughter cells. In conclusion, the present study showed that the particles can adhere to the cell surface, favoring their internalization. The presence of FBS contributed to the formation of smaller aggregates of particles, avoiding the formation of large ones, and thus transmitted a more efficient internalization of the materials through the interaction of the particles with the cell membrane.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionDepartment of Cell and Developmental Biology Institute of Biomedical Sciences University of São Paulo-
Descrição: dc.descriptionCentre of Excellence in New Target Discovery (CENTD) Butantan Institute-
Descrição: dc.descriptionCentre of Natural and Human Sciences (CCNH) Federal University of ABC-
Descrição: dc.descriptionDepartment of Physics and Chemistry Paulista State University-
Descrição: dc.descriptionDepartment of Physics and Chemistry Paulista State University-
Descrição: dc.descriptionFAPESP: 2015/20397-8-
Descrição: dc.descriptionFAPESP: 2017/16893-5-
Descrição: dc.descriptionFAPESP: 2018/22882-9-
Descrição: dc.descriptionFAPESP: 2019/21841-0-
Descrição: dc.descriptionCNPq: 316504/2021-1-
Descrição: dc.descriptionCAPES: 33002010024P6-
Idioma: dc.languageen-
Relação: dc.relationPharmaceutics-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectbreast cancer cell culture-
Palavras-chave: dc.subjectgraphene-
Palavras-chave: dc.subjectnanoparticles internalization-
Palavras-chave: dc.subjectreal-time microscopy-
Título: dc.titleInteraction of Graphene Oxide Particles and Dendrimers with Human Breast Cancer Cells by Real-Time Microscopy-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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