Metalloproteomic Investigation of Hg-Binding Proteins in Renal Tissue of Rats Exposed to Mercury Chloride

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversity of Nebraska-
Autor(es): dc.contributorLouisiana State University Health Sciences Center (LSUHSC)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorde Almeida, Emerson Carlos-
Autor(es): dc.creatorFaria, Victor Diego-
Autor(es): dc.creatorCirinêu, Felipe Dalmazzo-
Autor(es): dc.creatorSantiago, Maria G. A.-
Autor(es): dc.creatorMiotto, Beatriz-
Autor(es): dc.creatorVieira, José C. S.-
Autor(es): dc.creatorBraga, Camila Pereira-
Autor(es): dc.creatorAdamec, Jiri-
Autor(es): dc.creatorFernandes, Ana A. H.-
Autor(es): dc.creatorBuzalaf, Marília A. R.-
Autor(es): dc.creatorPadilha, Pedro de Magalhães-
Data de aceite: dc.date.accessioned2025-08-21T22:46:34Z-
Data de disponibilização: dc.date.available2025-08-21T22:46:34Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/ijms25010164-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/308364-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/308364-
Descrição: dc.descriptionResults obtained from rat studies indicate that, even at low concentrations, mercurial species cause harmful effects on the kidneys, by inducing the nephrotic oxidative stress response. In the present work, Hg-associated proteins were identified as possible mercury-exposure biomarkers in rat kidneys exposed to low mercury chloride concentrations for 30 days (Hg-30) and 60 days (Hg-60), using metalloproteomic strategies. The renal proteomic profile was fractioned by two-dimensional electrophoresis and the mercury determinations in kidney samples, protein pellets and protein spots were performed using graphite furnace atomic absorption spectrometry. The characterization of Hg-associated protein spots and the analysis of differentially expressed proteins were performed by liquid chromatography, coupled with tandem mass spectrometry. Eleven Hg-associated protein spots with a concentration range of 79 ± 1 to 750 ± 9 mg kg−1 in the Hg-60 group were identified. The characterization and expression analyses allowed the identification of 53 proteins that were expressed only in the Hg-60 group, 13 “upregulated” proteins (p > 0.95) and 47 “downregulated” proteins (p < 0.05). Actin isoforms and hemoglobin subunits were identified in protein spots of the Hg-60 group, with mercury concentrations in the range of 138 to 750 mg kg−1, which qualifies these proteins as potential mercury-exposure biomarkers.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionInstitute of Biosciences São Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Redox Biology University of Nebraska-
Descrição: dc.descriptionSchool of Medicine Louisiana State University Health Sciences Center (LSUHSC)-
Descrição: dc.descriptionFaculty of Dentistry of Bauru (FOB) University of Sao Paulo (USP), SP-
Descrição: dc.descriptionInstitute of Biosciences São Paulo State University (UNESP), SP-
Descrição: dc.descriptionCNPq: 30478/2018-9-
Descrição: dc.descriptionCNPq: 306440/2022-9-
Descrição: dc.descriptionCNPq: 404485/2016-2-
Descrição: dc.descriptionCNPq: 408179/2022-
Idioma: dc.languageen-
Relação: dc.relationInternational Journal of Molecular Sciences-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectLC-MS/MS-
Palavras-chave: dc.subjectmercurial species-
Palavras-chave: dc.subjectmercury bioaccumulation-
Palavras-chave: dc.subjectmercury biomarkers-
Palavras-chave: dc.subjectmetallomics-
Título: dc.titleMetalloproteomic Investigation of Hg-Binding Proteins in Renal Tissue of Rats Exposed to Mercury Chloride-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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