Enteroaggregative Escherichia coli (EAEC) isolates obtained from non-diarrheic children carry virulence factor-encoding genes from Extraintestinal Pathogenic E. Coli (ExPEC)

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorInstituto Adolfo Lutz-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorLuiz, Bruna M.-
Autor(es): dc.creatorCergole-Novella, Maria C.-
Autor(es): dc.creatorDantas, Stéfani T. A.-
Autor(es): dc.creatorde Lira, Daiany R. P.-
Autor(es): dc.creatorde Souza, Guilherme F. R.-
Autor(es): dc.creatorFernandes, Iranildo do A.-
Autor(es): dc.creatorOrsi, Henrique-
Autor(es): dc.creatorSolveira, Guilherme-
Autor(es): dc.creatorRall, Vera L. M.-
Autor(es): dc.creatordos Santos, Luís F.-
Autor(es): dc.creatorHernandes, Rodrigo T.-
Data de aceite: dc.date.accessioned2025-08-21T20:56:11Z-
Data de disponibilização: dc.date.available2025-08-21T20:56:11Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s42770-024-01471-2-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/308317-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/308317-
Descrição: dc.descriptionEnteroaggregative E. coli (EAEC) is one of the most frequent pathogens isolated from diarrheal patients as well as from healthy individuals in Brazil and has recently also been implicated as an extraintestinal pathogenic E. coli (ExPEC) associated with bloodstream and urinary tract infections. In this study, 37 EAEC isolates, obtained from fecal samples of non-diarrheic children, were molecularly and phenotypically characterized to access the pathogenic features of these isolates. The EAEC isolates were assigned into the phylogroups A (54.1%), D (29.7%), B1 (13.5%) and B2 (2.7%); and harbored genes responsible for encoding the major pilin subunit of the aggregative adherence fimbriae (AAFs) or aggregate-forming pili (AFP) adhesins as follows: aggA (24.3%), agg3A (5.4%), agg4A (27.0%), agg5A (32.4%) and afpA (10.8%). The most frequent O:H serotypes were O15:H2 (8.1%), O38:H25 (5.4%) and O86:H2 (5.4%). Twenty-one isolates (56.8%) produce the aggregative adherence (AA) pattern on HeLa cells, and biofilm formation was more efficient among EAEC isolates harboring the aggA and agg5A genes. PFGE analysis showed that 31 (83.8%) of the isolates were classified into 10 distinct clusters, which reinforces the high diversity found among the isolates studied. Of note, 40.5% (15/37) of the EAEC isolates have a genetic profile compatible with E. coli isolates with intrinsic potential to cause extraintestinal infections in healthy individuals, and therefore, classified as EAEC/ExPEC hybrids. In conclusion, we showed the presence of EAEC/ExPEC hybrids in the intestinal microbiota of non-diarrheic children, possibly representing the source of some endogenous extraintestinal infections.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionInstituto de Biociências Universidade Estadual Paulista (UNESP), SP-
Descrição: dc.descriptionLaboratório Regional de Santo André Instituto Adolfo Lutz, SP-
Descrição: dc.descriptionFaculdade de Medicina Veterinária e Zootecnia Universidade de São Paulo, SP-
Descrição: dc.descriptionCentro de Bacteriologia Instituto Adolfo Lutz, SP-
Descrição: dc.descriptionInstituto de Biociências Universidade Estadual Paulista (UNESP), SP-
Descrição: dc.descriptionFAPESP: 2017/14821-7-
Formato: dc.format3551-3561-
Idioma: dc.languageen-
Relação: dc.relationBrazilian Journal of Microbiology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectEAEC-
Palavras-chave: dc.subjectExPEC-
Palavras-chave: dc.subjectHybrid E. Coli isolates-
Título: dc.titleEnteroaggregative Escherichia coli (EAEC) isolates obtained from non-diarrheic children carry virulence factor-encoding genes from Extraintestinal Pathogenic E. Coli (ExPEC)-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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