Titanium dioxide nanotubes applied to conventional glass ionomer cement influence the expression of immunoinflammatory markers: An in vitro study

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorFaculdade São Leopoldo Mandic (SLMANDIC)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorUniversity of Kentucky-
Autor(es): dc.contributorInnovation and Technology Research-
Autor(es): dc.creatorRangel-Coelho, João Pedro-
Autor(es): dc.creatorGogolla, Pedro Viel-
Autor(es): dc.creatorMeyer, Maria Davoli-
Autor(es): dc.creatorSimão, Lucas Carvalho-
Autor(es): dc.creatorCosta, Bruna Carolina-
Autor(es): dc.creatorCasarin, Renato Côrrea Viana-
Autor(es): dc.creatorSantamaria, Mauro Pedrine-
Autor(es): dc.creatorTeixeira, Lucas Novaes-
Autor(es): dc.creatorPeruzzo, Daiane Cristina-
Autor(es): dc.creatorLisboa-Filho, Paulo Noronha-
Autor(es): dc.creatorNociti-Jr, Francisco Humberto-
Autor(es): dc.creatorKantovitz, Kamila Rosamilia-
Data de aceite: dc.date.accessioned2025-08-21T19:42:35Z-
Data de disponibilização: dc.date.available2025-08-21T19:42:35Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-05-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.heliyon.2024.e30834-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/308081-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/308081-
Descrição: dc.descriptionObjectives: To assess the impact of different concentrations TiO2-nt incorporated into a glass ionomer cement on the proliferation, mitochondrial metabolism, morphology, and pro- and anti-inflammatory cytokine production of cultured fibroblasts (NIH/3T3), whether or not stimulated by lipopolysaccharides (LPS-2 μg/mL, 24 h). Methods: TiO2-nt was added to KM (Ketac Molar EasyMix™, 3 %, 5 %, 7 % in weight); unblended KM was used as the control. The analyses included: Cell proliferation assay (n = 6; 24/48/72h); Mitochondrial metabolism assay (n = 6; 24/48/72h); Confocal laser microscopy (n = 3; 24/48/72h); Determination of biomarkers (IL-1β/IL-6/IL-10/VEGF/TNF) by using both multiplex technology (n = 6; 12/18 h) and the quantitative real-time PCR assay (q-PCR) (n = 3, 24/72/120 h). The data underwent analysis using both the Shapiro-Wilk and Levene tests, and by generalized linear models (α = 0.05). Results: It demonstrated that cell proliferation increased over time, regardless of the presence of TiO2-nt or LPS, and displayed a significant increase at 72 h; mitochondrial metabolism increased (p < 0.05), irrespective of exposure to LPS (p = 0.937); no cell morphology changes were observed; TiO2-nt reverted the impact of KM on the secreted levels of the evaluated proteins and the gene expressions in the presence of LPS (p < 0.0001). Conclusions: TiO2-nt did not adversely affect the biological behavior of fibroblastic cells cultured on GIC discs.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionFaculdade São Leopoldo Mandic (SLMANDIC), Rua José Rocha Junqueira 13, Swift-
Descrição: dc.descriptionDepartment of Physics School of Science State University Júlio de Mesquita (UNESP), Av. Engenheiro Luís Edmundo Carrijo Coube 2085-
Descrição: dc.descriptionDepartment of Prosthodontics and Periodontics Division of Periodontics Piracicaba Dental School State University of Campinas (FOP-UNICAMP), Av. Limeira 901, Areião-
Descrição: dc.descriptionCollege of Dentistry University of Kentucky, 1095 Veterans Dr-
Descrição: dc.descriptionAmerican Dental Association Science and Research Institute - ADASRI Cellular and Molecular Biology Research Group Innovation and Technology Research, 100 Bureau Dr-
Descrição: dc.descriptionDepartment of Physics School of Science State University Júlio de Mesquita (UNESP), Av. Engenheiro Luís Edmundo Carrijo Coube 2085-
Descrição: dc.descriptionFAPESP: #2019/07363-8-
Descrição: dc.descriptionFAPESP: #2019/14078-8-
Descrição: dc.descriptionFAPESP: #2022/04218-0-
Descrição: dc.descriptionFAPESP: #2024/05610-6-
Idioma: dc.languageen-
Relação: dc.relationHeliyon-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectFibroblasts-
Palavras-chave: dc.subjectGlass ionomer cements-
Palavras-chave: dc.subjectInterleukins-
Palavras-chave: dc.subjectNanotechnology-
Palavras-chave: dc.subjectProteome-
Palavras-chave: dc.subjectTitanium dioxide-
Título: dc.titleTitanium dioxide nanotubes applied to conventional glass ionomer cement influence the expression of immunoinflammatory markers: An in vitro study-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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