Oxygen Availability on the Application of Antimicrobial Photodynamic Therapy against Multi-Species Biofilms

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Autor(es): dc.contributorGuangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine-
Autor(es): dc.contributorSichuan University-
Autor(es): dc.contributorUniversity of Amsterdam and Vrije Universiteit Amsterdam-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorNie, Min-
Autor(es): dc.creatorYang, Jingmei-
Autor(es): dc.creatorRastelli, Alessandra Nara de Souza-
Autor(es): dc.creatorShen, Yuqin-
Autor(es): dc.creatorDeng, Dongmei-
Data de aceite: dc.date.accessioned2025-08-21T16:08:40Z-
Data de disponibilização: dc.date.available2025-08-21T16:08:40Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-07-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/pathogens12070904-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/308053-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/308053-
Descrição: dc.descriptionMethylene blue (MB)- or Curcumin (Cur)-based photodynamic therapy (PDT) has been used as an adjunctive treatment for periodontitis. Its actual clinical efficacy is still in question because the lack of oxygen in a deep periodontal pocket might reduce the PDT efficacy. We aim to investigate the effect of oxygen on PDT efficacy and to examine if the addition of hydrogen peroxide (HP) could improve PDT performance anaerobically. To this end, we cultured 48 h saliva-derived multi-species biofilms and treated the biofilms with 25 µM MB or 40 µM Cur, HP (0.001%, 0.01% and 0.1%), light (L-450 nm or L-660 nm), or combinations thereof under ambient air or strictly anaerobic conditions. MB- and Cur-PDTs significantly reduced biofilm viability in air but not under anaerobic conditions. HP at 0.1% significantly enhanced the killing efficacies of both MB- and Cur-PDTs anaerobically. The killing efficacy of Cur-PDT combined with 0.1% HP was higher anaerobically than in air. However, this was not the case for MB-PDT combined with 0.1% HP. In conclusion, this study demonstrated that the biofilm killing efficacies of MB- and Cur-PDTs diminished when there was no oxygen. HP at 0.1% can enhance the efficacy of PDT performed anaerobically, but the level of enhancement is photosensitizer-dependent.-
Descrição: dc.descriptionSichuan Province Science and Technology Support Program-
Descrição: dc.descriptionDepartment of Periodontics Affiliated Stomatology Hospital of Guangzhou Medical University Guangdong Engineering Research Center of Oral Restoration and Reconstruction Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine-
Descrição: dc.descriptionState Key Laboratory of Oral Disease & National Clinical Research Center for Oral Diseases Department of Periodontics West China Hospital of Stomatology Sichuan University-
Descrição: dc.descriptionDepartment of Preventive Dentistry Academic Centre for Dentistry Amsterdam University of Amsterdam and Vrije Universiteit Amsterdam-
Descrição: dc.descriptionDepartment of Restorative Dentistry School of Dentistry Sao Paulo State University-UNESP-
Descrição: dc.descriptionDepartment of Restorative Dentistry School of Dentistry Sao Paulo State University-UNESP-
Descrição: dc.descriptionSichuan Province Science and Technology Support Program: 2022NSFSC1521-
Idioma: dc.languageen-
Relação: dc.relationPathogens-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectbiofilm-
Palavras-chave: dc.subjectcurcumin-
Palavras-chave: dc.subjectmethylene blue-
Palavras-chave: dc.subjectoxygen-
Palavras-chave: dc.subjectphotodynamic therapy-
Título: dc.titleOxygen Availability on the Application of Antimicrobial Photodynamic Therapy against Multi-Species Biofilms-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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