Final irrigation with bioglass solution in regenerative endodontic procedure induces tissue formation inside the root canals, collagen maturation, proliferation cell and presence of osteocalcin

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Federal de Minas Gerais (UFMG)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.creatorde Paula, Kiani dos Santos-
Autor(es): dc.creatordos Reis-Prado, Alexandre Henrique-
Autor(es): dc.creatorde Jesus, Witalo Pereira-
Autor(es): dc.creatorGoto, Juliana-
Autor(es): dc.creatorde Arantes, Lara Cancella-
Autor(es): dc.creatorVerçosa, Marina-
Autor(es): dc.creatorCintra, Luciano Tavares Angelo-
Autor(es): dc.creatorErvolino, Edilson-
Autor(es): dc.creatorSzawka, Raphael Escorsim-
Autor(es): dc.creatorCrovace, Murilo Camuri-
Autor(es): dc.creatorde Mesquita, Ricardo Alves-
Autor(es): dc.creatorBenetti, Francine-
Data de aceite: dc.date.accessioned2025-08-21T21:22:31Z-
Data de disponibilização: dc.date.available2025-08-21T21:22:31Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-05-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1111/iej.14033-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/307873-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/307873-
Descrição: dc.descriptionAim: To evaluate the influence of an experimental solution of cobalt-doped F18 bioactive glass (F18Co) on tissue repair following regenerative endodontic procedure (REP) in rat molars. Methodology: The F18Co solution was prepared at a ratio of 1:5 F18Co powder to distilled water. The right or left upper first molars of 12 Wistar rats were used, where the pulps were exposed, removed, and irrigated with 2.5% sodium hypochlorite (NaOCl), followed by 17% ethylenediaminetetraacetic acid (EDTA) (5 min each). Subsequently, the molars were divided into two groups (n = 6): REP-SS and REP-F18Co, where they received a final irrigation (5 min) with saline solution (SS) or F18Co solution, respectively. Then, intracanal bleeding was induced, and the tooth was sealed. Untreated molars were used as controls (n = 3). At 21 days, the rats were euthanized, and the specimens were processed for analysis of mineralized tissue and soft tissue formation inside the root canal using haematoxylin-eosin. The presence and maturation of collagen were evaluated by Masson's trichrome and picrosirius red staining. Immunolabelling analyses of proliferating cell nuclear antigen (PCNA) and osteocalcin (OCN) were performed. The data were submitted to the Mann–Whitney U-test (p <.05). Results: There was a similar formation of mineralized tissue in thickness and length in REP-SS and REP-F18Co groups (p >.05). Regarding the presence of newly formed soft tissue, most specimens of the REP-F18Co had tissue formation up to the cervical third of the canal, whilst the REP-SS specimens showed formation up to the middle third (p <.05), and there was higher maturation of collagen in REP-F18Co (p <.05). The number of PCNA-positive cells found in the apical third of the root canal was significantly higher in the F18Co group, as well as the OCN immunolabelling, which was severe in most specimens of REP-F18Co, and low in most specimens of REP-SS. Conclusion: The final irrigation with F18Co bioactive glass solution in REP did not influence mineralized tissue formation but induced soft tissue formation inside the root canals, with higher collagen maturation, and an increase in PCNA-positive cells and OCN immunolabelling.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionDepartment of Restorative Dentistry Universidade Federal de Minas Gerais (UFMG) School of Dentistry-
Descrição: dc.descriptionDepartment of Restorative Dentistry São Paulo State University (UNESP) School of Dentistry-
Descrição: dc.descriptionDepartment of Basic Science São Paulo State University (UNESP) School of Dentistry-
Descrição: dc.descriptionPhysiology and Biophysics Institute of Biological Sciences Universidade Federal de Minas Gerais (UFMG)-
Descrição: dc.descriptionDepartment of Materials Engineering State University of São Carlos (UFSCar) Bioactive Materials Laboratory (LMBio)-
Descrição: dc.descriptionOral Pathology and Surgery Universidade Federal de Minas Gerais (UFMG) School of Dentistry-
Descrição: dc.descriptionDepartment of Restorative Dentistry São Paulo State University (UNESP) School of Dentistry-
Descrição: dc.descriptionDepartment of Basic Science São Paulo State University (UNESP) School of Dentistry-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionCNPq: 310683/2022-0-
Formato: dc.format586-600-
Idioma: dc.languageen-
Relação: dc.relationInternational Endodontic Journal-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectbioactive glass-
Palavras-chave: dc.subjectbiocompatibility-
Palavras-chave: dc.subjectosteocalcin-
Palavras-chave: dc.subjectPCNA-
Palavras-chave: dc.subjectregenerative endodontic procedure-
Título: dc.titleFinal irrigation with bioglass solution in regenerative endodontic procedure induces tissue formation inside the root canals, collagen maturation, proliferation cell and presence of osteocalcin-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.