Metabolic engineering of Saccharomyces cerevisiae for second-generation ethanol production from xylo-oligosaccharides and acetate

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Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversity of Illinois at Urbana-Champaign-
Autor(es): dc.contributorUniversity of Illinois at Urbana-Champaign (UIUC)-
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorUniversity of Sorocaba (UNISO)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversity of Bath-
Autor(es): dc.contributorKorea Research Institute of Bioscience and Biotechnology-
Autor(es): dc.creatorProcópio, Dielle Pierotti-
Autor(es): dc.creatorLee, Jae Won-
Autor(es): dc.creatorShin, Jonghyeok-
Autor(es): dc.creatorTramontina, Robson-
Autor(es): dc.creatorÁvila, Patrícia Felix-
Autor(es): dc.creatorBrenelli, Lívia Beatriz-
Autor(es): dc.creatorSquina, Fabio Márcio-
Autor(es): dc.creatorDamasio, André-
Autor(es): dc.creatorRabelo, Sarita Cândida-
Autor(es): dc.creatorGoldbeck, Rosana-
Autor(es): dc.creatorFranco, Telma Teixeira-
Autor(es): dc.creatorLeak, David-
Autor(es): dc.creatorJin, Yong-Su-
Autor(es): dc.creatorBasso, Thiago Olitta-
Data de aceite: dc.date.accessioned2025-08-21T22:42:43Z-
Data de disponibilização: dc.date.available2025-08-21T22:42:43Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1038/s41598-023-46293-8-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/307862-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/307862-
Descrição: dc.descriptionSimultaneous intracellular depolymerization of xylo-oligosaccharides (XOS) and acetate fermentation by engineered Saccharomyces cerevisiae offers significant potential for more cost-effective second-generation (2G) ethanol production. In the present work, the previously engineered S. cerevisiae strain, SR8A6S3, expressing enzymes for xylose assimilation along with an optimized route for acetate reduction, was used as the host for expressing two β-xylosidases, GH43-2 and GH43-7, and a xylodextrin transporter, CDT-2, from Neurospora crassa, yielding the engineered SR8A6S3-CDT-2-GH34-2/7 strain. Both β-xylosidases and the transporter were introduced by replacing two endogenous genes, GRE3 and SOR1, that encode aldose reductase and sorbitol (xylitol) dehydrogenase, respectively, and catalyse steps in xylitol production. The engineered strain, SR8A6S3-CDT-2-GH34-2/7 (sor1Δ gre3Δ), produced ethanol through simultaneous XOS, xylose, and acetate co-utilization. The mutant strain produced 60% more ethanol and 12% less xylitol than the control strain when a hemicellulosic hydrolysate was used as a mono- and oligosaccharide source. Similarly, the ethanol yield was 84% higher for the engineered strain using hydrolysed xylan, compared with the parental strain. Xylan, a common polysaccharide in lignocellulosic residues, enables recombinant strains to outcompete contaminants in fermentation tanks, as XOS transport and breakdown occur intracellularly. Furthermore, acetic acid is a ubiquitous toxic component in lignocellulosic hydrolysates, deriving from hemicellulose and lignin breakdown. Therefore, the consumption of XOS, xylose, and acetate expands the capabilities of S. cerevisiae for utilization of all of the carbohydrate in lignocellulose, potentially increasing the efficiency of 2G biofuel production.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Chemical Engineering Escola Politécnica Universidade de São Paulo (USP), SP-
Descrição: dc.descriptionDOE Center for Advanced Bioenergy and Bioproducts Innovation (CABER) University of Illinois at Urbana-Champaign-
Descrição: dc.descriptionDepartment of Food Science and Human Nutrition University of Illinois at Urbana-Champaign (UIUC)-
Descrição: dc.descriptionDepartment of Biochemistry and Tissue Biology Institute of Biology University of Campinas (UNICAMP), SP-
Descrição: dc.descriptionEnvironment and Technological Processes Program University of Sorocaba (UNISO), SP-
Descrição: dc.descriptionSchool of Food Engineering University of Campinas (UNICAMP), SP-
Descrição: dc.descriptionInterdisciplinary Centre of Energy Planning University of Campinas (UNICAMP), SP-
Descrição: dc.descriptionDepartament of Bioprocesses and Biotechnology School of Agriculture Sao Paulo State University (UNESP), SP-
Descrição: dc.descriptionSchool of Chemical Engineering University of Campinas (UNICAMP), SP-
Descrição: dc.descriptionDepartment of Biology and Biochemistry University of Bath, Claverton Down-
Descrição: dc.descriptionDepartamento de Química Fundamental Instituto de Química Universidade de São Paulo), SP-
Descrição: dc.descriptionSynthetic Biology & Bioengineering Research Center Korea Research Institute of Bioscience and Biotechnology-
Descrição: dc.descriptionDepartament of Bioprocesses and Biotechnology School of Agriculture Sao Paulo State University (UNESP), SP-
Descrição: dc.descriptionFAPESP: #2015/50590-4-
Descrição: dc.descriptionFAPESP: #2015/50612-8-
Descrição: dc.descriptionFAPESP: #2017/15477-8-
Descrição: dc.descriptionFAPESP: #2018/01759-4-
Descrição: dc.descriptionFAPESP: #2018/17172-2-
Descrição: dc.descriptionFAPESP: #2021/04254-3-
Idioma: dc.languageen-
Relação: dc.relationScientific Reports-
???dc.source???: dc.sourceScopus-
Título: dc.titleMetabolic engineering of Saccharomyces cerevisiae for second-generation ethanol production from xylo-oligosaccharides and acetate-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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