Occurrence of Salmonella spp. in fecal samples from foals with and without diarrhea in the state of São Paulo: microbiological diagnosis, antimicrobial susceptibility profile, and molecular detection

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorCentro de Bacteriologia-
Autor(es): dc.contributorInstituto Biológico-
Autor(es): dc.creatorBraga, Pollyana R.C.-
Autor(es): dc.creatorBasso, Roberta M.-
Autor(es): dc.creatorMartins, Lorrayne S.A.-
Autor(es): dc.creatorRibeiro, Marcio G.-
Autor(es): dc.creatorAmarante, Ariadne F.-
Autor(es): dc.creatorCasas, Monique R.T.-
Autor(es): dc.creatorNassar, Alessandra F.C.-
Autor(es): dc.creatorPereira, Juliano G.-
Autor(es): dc.creatorPantoja, José Carlos F.-
Autor(es): dc.creatorCerri, Fabricio M.-
Autor(es): dc.creatorPossebon, Fábio S.-
Autor(es): dc.creatorOliveira-Filho, José P.-
Autor(es): dc.creatorBorges, Alexandre S.-
Data de aceite: dc.date.accessioned2025-08-21T23:45:46Z-
Data de disponibilização: dc.date.available2025-08-21T23:45:46Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2022-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1590/1678-5150-PVB-7194-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/307819-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/307819-
Descrição: dc.descriptionThe present study investigated Salmonella spp. in the feces of 200 foals up to one year of age (100 with clinical signs of diarrhea and 100 without clinical signs of diarrhea). Bacteriological culture, serotyping, antimicrobial susceptibility, and real-time PCR (qPCR SYBR® Green or a TaqMan®) for detecting the invA gene (with and without a selective pre-enrichment step in tetrathionate broth) were performed. Bacterial culture revealed 15% (n=30) of positive animals (21 animals with diarrhea and nine without diarrhea). Among the 30 isolates, 13 different serovars were identified: S. Infantis, S. Minnesota, S. I.4,5,12:i:-; S. Anatum, S. Cerro, S. Oranienburg, S. Braenderup, S. Give, S. Newport, S. IIIb 61:c:z35, S. 109:-:1.5, S. I.4.12:d:-, S. I.6.8:-:-. Multidrug resistance was found in 43.33% (n=13) of the isolates, with one isolate obtained from animals without diarrhea and 12 isolates from animals with diarrhea. All qPCR techniques used in the study classified more samples as positive for Salmonella spp. than the bacterial culture of feces. In addition, all qPCR techniques detected more positive animals in the diarrhea group than in the diarrhea-free group. The results confirm the utility of the qPCR method without the pre-enrichment step in tetrathionate as a rapid test for Salmonella spp. in carrier animals. In animals with clinical signs of diarrhea, it can be combined with bacterial culture (antimicrobial susceptibility testing and serotyping). The isolation of Salmonella spp. in nine animals without diarrhea confirms the importance of asymptomatic carrier animals in the epidemiology of the disease. The multidrug resistance observed highlights the importance of rational antimicrobial use in horses and adopting biosecurity protocols that are efficacious in controlling the spread of infections between animals and zoonotic transmission in farms.-
Descrição: dc.descriptionUniversidade Estadual Paulista Júlio de Mesquita Filho, Rua Prof. Dr. Walter Maurício Correa s/n, SP-
Descrição: dc.descriptionInstituto Adolfo Lutz Centro de Bacteriologia, Av. Doutor Arnaldo 351, SP-
Descrição: dc.descriptionInstituto Biológico, Rua Conselheiro Rodrigues Alves 1252, SP-
Descrição: dc.descriptionUniversidade Estadual Paulista Júlio de Mesquita Filho, Rua Prof. Dr. Walter Maurício Correa s/n, SP-
Idioma: dc.languageen-
Relação: dc.relationPesquisa Veterinaria Brasileira-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectfaeces-
Palavras-chave: dc.subjectHorses-
Palavras-chave: dc.subjectinvA gene-
Palavras-chave: dc.subjectmultidrug resistance-
Palavras-chave: dc.subjectSalmonella spp-
Palavras-chave: dc.subjectserovars-
Título: dc.titleOccurrence of Salmonella spp. in fecal samples from foals with and without diarrhea in the state of São Paulo: microbiological diagnosis, antimicrobial susceptibility profile, and molecular detection-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.