Dhvar5- and MSI78-coated titanium are bactericidal against methicillin-resistant Staphylococcus aureus, immunomodulatory and osteogenic

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Autor(es): dc.contributorUniversidade do Porto-
Autor(es): dc.contributorColégio Internato dos Carvalhos (CIC)-
Autor(es): dc.contributorUniversity of Navarra-
Autor(es): dc.contributorNavarra Institute for Health Research (IdiSNA)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorInternational Iberian Nanotechnology Laboratory-
Autor(es): dc.creatorCosta, B.-
Autor(es): dc.creatorCoelho, J.-
Autor(es): dc.creatorSilva, V.-
Autor(es): dc.creatorShahrour, H.-
Autor(es): dc.creatorCosta, N. A.-
Autor(es): dc.creatorRibeiro, A. R.-
Autor(es): dc.creatorSantos, S. G.-
Autor(es): dc.creatorCosta, F.-
Autor(es): dc.creatorMartínez-de-Tejada, G.-
Autor(es): dc.creatorMonteiro, C.-
Autor(es): dc.creatorMartins, M. C.L.-
Data de aceite: dc.date.accessioned2025-08-21T15:17:39Z-
Data de disponibilização: dc.date.available2025-08-21T15:17:39Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.actbio.2024.11.016-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/307770-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/307770-
Descrição: dc.descriptionInfection is one of the major issues associated with the failure of orthopedic devices, mainly due to implant bacterial colonization, biofilm formation, and associated antibiotic resistance. Antimicrobial peptides (AMP) are a promising alternative to conventional antibiotics given their broad-spectrum of activity, low propensity to induce bacterial resistance, and ability to modulate host immune responses. Dhvar5 (LLLFLLKKRKKRKY) and MSI78 (GIGKFLKKAKKFGKAFVKILKK) are two AMP with broad-spectrum activity against bacteria, including methicillin-resistant Staphylococcus aureus (MRSA), one of the most problematic etiologic agents in Orthopedic Devices-Related Infections (ODRI). This work aims to evaluate the bactericidal, immunomodulatory and osteogenic potential of Dhvar5- and MSI78-coated titanium surfaces (AMP-Ti). Two AMP-Ti surfaces were successfully obtained by grafting Dhvar5 (0.8 ± 0.1 µM/mm2) or MSI78 (0.5 ± 0.3 µM/mm2) onto titanium substrates through a polydopamine layer. Both AMP-Ti were bactericidal against MRSA, eradicating bacteria upon contact for 6 h in a culture medium supplemented with human plasma proteins. The AMP-Ti immunomodulatory potential was evaluated using human primary macrophages, by assessing surfaces capacity to induce pro-/anti-inflammatory (M1/M2) markers and cytokines. While in naïve conditions both AMP-Ti surfaces slightly promoted the M2 marker CD163, in response to LPS and IFN-γ (simulating a bacterial infection), both AMP increased the M1 marker CCR7 and enhanced macrophage secretion of pro-inflammatory IL-6 and TNF-α cytokines, particularly for Ti-MSI78 surfaces. Additionally, both AMP-Ti surfaces were cytocompatible and promoted osteoblastic cell differentiation. This proof-of-concept study demonstrated the high potential of Dhvar5- and MSI78-Ti as antimicrobial coatings for ODRI prevention. Statement of significance: This study investigates the bactericidal effects of the antimicrobial peptides Dhvar5 and MSI78, immobilized on titanium (Ti) surfaces through a polydopamine coating, aiming at the prevention of Orthopedic-Device Related Infections (ODRIs). The developed coatings displayed MRSA-sterilizing activity, while revealing an immunomodulatory potential towards macrophages and promoting osteoblastic cell differentiation. This strategy allows a quick and easy immobilization of high quantities of AMP, unlike most other approaches, thus favoring its clinical translation.-
Descrição: dc.descriptioni3S – Instituto de Investigação e Inovação em Saúde Universidade do Porto-
Descrição: dc.descriptionINEB – Instituto de Engenharia Biomédica Universidade do Porto-
Descrição: dc.descriptionFEUP–Faculdade de Engenharia Universidade do Porto-
Descrição: dc.descriptionICBAS – Instituto de Ciências Biomédicas Abel Salazar Universidade do Porto-
Descrição: dc.descriptionColégio Internato dos Carvalhos (CIC)-
Descrição: dc.descriptionDepartment of Microbiology and Parasitology University of Navarra-
Descrição: dc.descriptionNavarra Institute for Health Research (IdiSNA)-
Descrição: dc.descriptionUNESP - Universidade Estadual Paulista Faculdade de Ciências, SP-
Descrição: dc.descriptionNanoSafety Group International Iberian Nanotechnology Laboratory-
Descrição: dc.descriptionUNESP - Universidade Estadual Paulista Faculdade de Ciências, SP-
Formato: dc.format98-112-
Idioma: dc.languageen-
Relação: dc.relationActa Biomaterialia-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAntibacterial activity-
Palavras-chave: dc.subjectAntimicrobial peptides-
Palavras-chave: dc.subjectDopamine-
Palavras-chave: dc.subjectImmunomodulation-
Palavras-chave: dc.subjectMacrophages-
Palavras-chave: dc.subjectSurface immobilization-
Título: dc.titleDhvar5- and MSI78-coated titanium are bactericidal against methicillin-resistant Staphylococcus aureus, immunomodulatory and osteogenic-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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