Antibiofilm properties, cytotoxicity, and effect on protease activity of antibiotics and EGCG-based medications for endodontic purposes

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorFaculty of Dentistry-
Autor(es): dc.contributorDivision of Biomaterials and Biomechanics-
Autor(es): dc.contributorCollege of Dentistry-
Autor(es): dc.contributorFaculty of Arts and Science-
Autor(es): dc.contributorCentre for Interdisciplinary Research in Health (CIIS)-
Autor(es): dc.creatorChrisostomo, Daniela Alvim-
Autor(es): dc.creatorPereira, Jesse Augusto-
Autor(es): dc.creatorScaffa, Polliana Mendes Candia-
Autor(es): dc.creatorGouveia, Zach-
Autor(es): dc.creatorAbuna, Gabriel Flores-
Autor(es): dc.creatorPlotnikov, Sergey V.-
Autor(es): dc.creatorPrakki, Anuradha-
Autor(es): dc.creatorDuque, Cristiane-
Data de aceite: dc.date.accessioned2025-08-21T21:54:50Z-
Data de disponibilização: dc.date.available2025-08-21T21:54:50Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2025-05-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.jdent.2025.105660-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/307746-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/307746-
Descrição: dc.descriptionObjective: To evaluate the effect of two intracanal medications (IM) containing epigallocatechin-3-gallate (EGCG) with fosfomycin (FOSFO) and a triantibiotic combination of metronidazole, ciprofloxacin and fosfomycin (TRI), compared to controls calcium hydroxide (CH), all dissolved in polyethylene glycol 400 (PEG) on multispecies biofilms, fibroblast toxicity and on collagenolytic and gelatinolytic activities detected in radicular dentin. Methods: The antibiofilm effect and cytotoxicity of medications containing EGCG + FOSFO, TRI or CH were evaluated on multispecies biofilms formed in bovine root dentin specimens by confocal microscopy and on fibroblasts by resazurin assays, respectively. The inhibition of protease activity of each IM was evaluated by measuring collagenolytic enzyme activity by ELISA (Enzyme-linked immunosorbent assay) and gelatinolytic activity by metalloproteinases (MMPs) using in situ zymography in radicular dentin specimens. Results: PEG containing EGCG+FOSFO, PEG+TRI, and PEG+CH significantly reduced multispecies biofilms in radicular dentin tubules. At the concentrations tested, those IM were not toxic to fibroblasts. Additionally, all IM presented anti-collagenolytic activity by reducing telopeptide fragments released from radicular dentin compared to PEG carrier and water controls. In situ gelatinolytic activity, assessed via fluorescence levels, was significantly lower in radicular dentin adjacent to PEG containing CH, EGCG+FOSFO, or TRI compared to PEG and water controls. Conclusion: EGCG+FOSFO and TRI in PEG-400 exhibited antibiofilm, anti-collagenolytic and anti-gelatinolytic properties at concentrations that were non-toxic to fibroblasts, making them feasible intracanal medications for endodontic applications. Clinical significance: EGCG-based medications enhance the efficacy of endodontic treatment by providing antibiofilm, anti-collagenolytic, and anti-gelatinolytic properties, contributing to the preservation of root structure and improved treatment outcomes.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionCanadian Institutes of Health Research-
Descrição: dc.descriptionNatural Sciences and Engineering Research Council of Canada-
Descrição: dc.descriptionSão Paulo State University (UNESP) Department of Preventive and Restorative Dentistry School of Dentistry, SP-
Descrição: dc.descriptionUniversity of Toronto Department of Clinical Sciences Faculty of Dentistry-
Descrição: dc.descriptionOregon Health & Science University (OHSU) Division of Biomaterials and Biomechanics Department of Restorative Dentistry-
Descrição: dc.descriptionNova Southeastern University College of Dentistry-
Descrição: dc.descriptionUniversity of Toronto Department of Cell and Systems Biology Faculty of Arts and Science-
Descrição: dc.descriptionUniversidade Católica Portuguesa (UCP) Faculty of Dental Medicine Centre for Interdisciplinary Research in Health (CIIS)-
Descrição: dc.descriptionSão Paulo State University (UNESP) Department of Preventive and Restorative Dentistry School of Dentistry, SP-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionCanadian Institutes of Health Research: PJT-178272-
Descrição: dc.descriptionNatural Sciences and Engineering Research Council of Canada: RGPIN-2020-05881-
Idioma: dc.languageen-
Relação: dc.relationJournal of Dentistry-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAntibiotics-
Palavras-chave: dc.subjectAntimicrobial-
Palavras-chave: dc.subjectBiofilms-
Palavras-chave: dc.subjectCollagen degradation-
Palavras-chave: dc.subjectEndodontics-
Palavras-chave: dc.subjectMetalloproteinases-
Título: dc.titleAntibiofilm properties, cytotoxicity, and effect on protease activity of antibiotics and EGCG-based medications for endodontic purposes-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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