Zerumbone enhances the photodynamic effect against biofilms of fluconazole-resistant Candida albicans clinical isolates

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorAbreu-Pereira, César Augusto-
Autor(es): dc.creatorGorayb-Pereira, Ana Luiza-
Autor(es): dc.creatorJordão, Cláudia Carolina-
Autor(es): dc.creatorParo, Clara Brait-
Autor(es): dc.creatorBarbugli, Paula Aboud-
Autor(es): dc.creatorPavarina, Ana Claudia-
Data de aceite: dc.date.accessioned2025-08-21T18:52:37Z-
Data de disponibilização: dc.date.available2025-08-21T18:52:37Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2025-04-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.jdent.2025.105631-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/307708-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/307708-
Descrição: dc.descriptionThe aim was the evaluation of Zerumbone (ZER) associated with Photodithazine® (PDZ) mediated antimicrobial photodynamic therapy (aPDT) on fluconazole-resistant (CaR) and -susceptible (CaS) Candida albicans biofilms, and well as two fluconazole-resistant clinical isolates (R70 and R14). Biofilms (48 h) were treated with ZER (256 µg/mL) for 20 min, followed by the absence or presence of the photosensitizer (PDZ; 200 mg/L) and Light Emitting Diode (LED; 600 nm; 50 Jcm2), either alone or in combination, resulting in 8 treatment groups (Control; ZER; aPDT; PDZ; LED; ZER+aPDT; ZER+PDZ; ZER+LED; n = 12). The treatments were evaluated by colony-forming unit (CFU), total and insoluble biomass, extracellular matrix components (ECM) quantification [extracellular DNA (eDNA), proteins, alkali (ASP) and water-soluble polysaccharides (WSP)], and Confocal Laser Scanning Microscopy (CLSM). Data were subjected to three-way ANOVA with Bonferroni post-test (α 5 %). The combination of ZER and aPDT promoted a greater reduction of biofilms’ components for all the strains evaluated (CaS, CaR, R14, R70). On average, ZER+aPDT group showed a 2.01 log10 (30 %) reduction in CFU counts, total biomass (30 %), insoluble biomass (33 %), and total protein (15 %). Additionally, there was a reduction in ECM components of the biofilms, such as insoluble proteins (24 %), WSP (68 %), ASP (26 %) and eDNA (60 %) compared to the control group. Images obtained through CLSM showed that the association of ZER+aPDT resulted in a higher content of dead cells. Treatment with ZER+aPDT enhanced the efficacy of photodynamic treatment on fluconazole-resistant C. albicans (including clinical isolates), representing an encouraging antifungal approach.-
Descrição: dc.descriptionLaboratory of Applied Microbiology Department of Dental Materials and Prosthodontics São Paulo State University (UNESP) School of Dentistry, São Paulo-
Descrição: dc.descriptionLaboratory of Applied Microbiology Department of Dental Materials and Prosthodontics São Paulo State University (UNESP) School of Dentistry, São Paulo-
Idioma: dc.languageen-
Relação: dc.relationJournal of Dentistry-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBiofilms-
Palavras-chave: dc.subjectCandida albicans-
Palavras-chave: dc.subjectPhotochemotherapy-
Palavras-chave: dc.subjectZerumbone-
Título: dc.titleZerumbone enhances the photodynamic effect against biofilms of fluconazole-resistant Candida albicans clinical isolates-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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