Influence of photoactivation on tissue response to different dyes used in photodynamic therapy and laser ablation therapy

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorSecretariat of the Mexican Navy-
Autor(es): dc.creatorCintra, Luciano Tavares Angelo-
Autor(es): dc.creatorCantiga-Silva, Cristiane-
Autor(es): dc.creatorBanci, Henrique Augusto-
Autor(es): dc.creatorFaria, Flávio Duarte-
Autor(es): dc.creatorda Silva Machado, Nathália Evelyn-
Autor(es): dc.creatorCardoso, Carolina de Barros Morais-
Autor(es): dc.creatorde Oliveira, Pedro Henrique Chaves-
Autor(es): dc.creatorEstrela, Lucas Rodrigues de Araújo-
Autor(es): dc.creatorSivieri-Araujo, Gustavo-
Autor(es): dc.creatorBerbert, Fabio Luiz Camargo Villela-
Autor(es): dc.creatorGarcia, Arturo Javier Aranda-
Autor(es): dc.creatorLeonardo, Renato de Toledo-
Data de aceite: dc.date.accessioned2025-08-21T16:36:51Z-
Data de disponibilização: dc.date.available2025-08-21T16:36:51Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-01-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.jphotobiol.2024.112843-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/307600-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/307600-
Descrição: dc.descriptionLaser ablation therapy (LA) uses Indocyanine Green dye (ICG) which efficiently absorbs laser energy and the increased temperature results in an instantaneous flame that chars tissue and microbes. Photodynamic therapy (PDT) uses different dyes that are activated by light to kill bacteria. This study evaluated the biocompatibility of the dye Curcumin (CUR), Methylene Blue (MB), and Indocyanine Green (ICG) before and after laser activation (ACT). Polyethylene tubes containing one of the dyes were implanted in the subcutaneous tissue of 32 rats (4 tubes per rat) which were divided into 8 groups: C - control (saline solution); C + ACT (Red Laser 660 nm); CUR; CUR + ACT (480 nm blue LED); MB; MB + ACT (Red Laser 660 nm); ICG; ICG + ACT (810 nm Infrared Laser). After 7 and 30 days (n = 8/time), the rats were euthanized and the tubes with the surrounding tissue were removed and processed for histological analysis of inflammation using H&E stain, and collagen fiber maturation using picrosirius red (PSR). A two-way analysis of variance statistical test was applied (p < 0.05). At 7 days, regardless of laser activation, the CUR group showed a greater inflammatory infiltrate compared to the ICG and control groups, and the MB group had a greater inflammation only in relation to the control (p < 0.05). At 30 days, CUR and MB groups showed a greater inflammatory infiltrate than the control (p < 0.05). ICG group was equal to the control in both periods, regardless of the laser activation (p > 0.05). Laser activation induced the proliferation of collagen immature fibers at 7 days, regardless of the dye (p < 0.05). The CUR group showed a lower percentage of immature and mature fibers at 7 days, compared to ICG and control (p < 0.05) and, at 30 days, compared to control (p < 0.05). Regardless of laser activation, the ICG showed the results of collagen maturation closest to the control (p > 0.05). It was concluded that all dyes are biocompatible and that laser activation did not interfere with biocompatibility. In addition, the maturity of collagen was adequate before and after the laser activation. These results demonstrate that the clinical use of dyes is safe even when activated with a laser.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionDepartment of Preventive and Restorative Dentistry São Paulo State University (UNESP) School of Dentistry, SP-
Descrição: dc.descriptionDepartment of Restorative Dentistry School of Dentistry São Paulo State University (UNESP), SP-
Descrição: dc.descriptionSecretariat of the Mexican Navy-
Descrição: dc.descriptionDepartment of Preventive and Restorative Dentistry São Paulo State University (UNESP) School of Dentistry, SP-
Descrição: dc.descriptionDepartment of Restorative Dentistry School of Dentistry São Paulo State University (UNESP), SP-
Descrição: dc.descriptionCNPq: 315381/2021-3-
Idioma: dc.languageen-
Relação: dc.relationJournal of Photochemistry and Photobiology B: Biology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBiocompatibility-
Palavras-chave: dc.subjectDyes-
Palavras-chave: dc.subjectLaser ablation-
Palavras-chave: dc.subjectLaser activation-
Palavras-chave: dc.subjectPhotodynamic therapy-
Título: dc.titleInfluence of photoactivation on tissue response to different dyes used in photodynamic therapy and laser ablation therapy-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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