Cytotoxicity evaluation of Chlorhexidine and Blue®M applied to a human gingival fibroblast (HGF-1) and keratinocytes (NOK-SI): In vitro study

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorPrivate Practice-
Autor(es): dc.creatorCunha, Giovanni-
Autor(es): dc.creatorD'Angieri Saugo, Gustavo-
Autor(es): dc.creatorGabrielli, Marisa Aparecida Cabrini-
Autor(es): dc.creatorBarbeiro, Camila de Oliveira-
Autor(es): dc.creatorde Almeida, Luciana Yamamoto-
Autor(es): dc.creatorBufalino, Andréia-
Autor(es): dc.creatorPereira-Filho, Valfrido Antônio-
Data de aceite: dc.date.accessioned2025-08-21T19:35:34Z-
Data de disponibilização: dc.date.available2025-08-21T19:35:34Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-10-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.jormas.2024.101923-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/307577-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/307577-
Descrição: dc.descriptionChlorhexidine (CHX) is a prime choice to control the oral microbiota. However, it's a chemical agent leading to side effects such as teeth strains, taste disturbance, and desquamation of oral mucosa. Alternatively, the lactoferrin and oxygen-based Blue®M has been introduced as an alternative to the CHX, not disturbing tissue repair. Therefore, the study aimed to evaluate the effects of Blue®M and CHX on oral human fibroblasts (HGF-1) and keratinocytes (NOK-SI). Cell cultures using HGF-1 and NOK-SI evaluated cell proliferation, cell cycle, apoptosis and necrosis, and migration. In the dose-effect test, Blue®M reduced the HGF-1 sample in a 4-fold concentration than CHX (CHX: 173.07 ±10.27; Blue®M: 43.86 ±3.04). The proliferation test revealed an eightfold reduction of the sample for CHX, while for Blue®M, the proliferation rate was eighteen times lower. The apoptosis and necrosis rates increased by 25% (p<0.0001) for HGF-1 for both substances. In NOK-SI, the apoptosis rates increased by 10% (p=0.02) and 15% (p=0.001) for CHX and Blue®M, respectively. Furthermore, the fibroblast had a lower capacity for wound closure in the Scratch Assay (monolayer cell migration) for Blue®M. Despite the limitations of this in vitro study, the results of the lactoferrin and oxygen-based Blue®M demonstrated cytotoxicity in doses over the Minimum inhibitory concentration and Minimum bactericidal concentration for Oral fibroblasts (HGF- 1) and Keratinocytes (NOK-SI).-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery Division of Oral and Maxillofacial Surgery São Paulo State University (Unesp) School of Dentistry-
Descrição: dc.descriptionPrivate Practice-
Descrição: dc.descriptionOral Medicine Department of Diagnosis and Surgery São Paulo State University (Unesp) School of Dentistry, SP-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery São Paulo State University (Unesp) School of Dentistry, SP-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery Division of Oral and Maxillofacial Surgery São Paulo State University (Unesp) School of Dentistry-
Descrição: dc.descriptionOral Medicine Department of Diagnosis and Surgery São Paulo State University (Unesp) School of Dentistry, SP-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery São Paulo State University (Unesp) School of Dentistry, SP-
Descrição: dc.descriptionFAPESP: FAPESP 2019/26431–4-
Idioma: dc.languageen-
Relação: dc.relationJournal of Stomatology, Oral and Maxillofacial Surgery-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBlue®M-
Palavras-chave: dc.subjectChlorhexidine-
Palavras-chave: dc.subjectOral surgery-
Palavras-chave: dc.subjectWound healing-
Título: dc.titleCytotoxicity evaluation of Chlorhexidine and Blue®M applied to a human gingival fibroblast (HGF-1) and keratinocytes (NOK-SI): In vitro study-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.