Antitumor activity of membranes associated with Acmella oleracea extract

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade do Vale do Paraíba-
Autor(es): dc.contributorUniversidade Federal de São Paulo (UNIFESP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorPriante-Silva, C. A.-
Autor(es): dc.creatorGodoi, B. H.-
Autor(es): dc.creatorMenegon, R. F.-
Autor(es): dc.creatorda Silva, N. S.-
Autor(es): dc.creatorPacheco-Soares, C.-
Data de aceite: dc.date.accessioned2025-08-21T16:50:51Z-
Data de disponibilização: dc.date.available2025-08-21T16:50:51Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1590/1414-431X2024e14129-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/307434-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/307434-
Descrição: dc.descriptionEpithelial cancers, such as epidermoid cancer and some adenocarcinomas, affect surface areas that are generally more accessible to various treatments. However, this group of tumor cells has an aggressive behavior, leading to a high annual mortality rate. The development of a biomaterial that is non-invasive, can kill tumor cells, and prevent opportunistic infections is the basis for the treatment for this type of cancer. Therefore, the objective of this study was to develop a biomaterial from chitosan and A. oleracea extracts that exhibits cytotoxic action against the HEp-2 tumor cell line. Dried crude 90% ethanol extracts were obtained through ultrasound-assisted maceration, followed by liquid-liquid extraction to yield the butanol fraction. From these extracts, chitosan membranes were developed and evaluated for their antitumor activity against HEp-2 using viability tests with crystal violet and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, in addition to a wound healing test. The cytotoxic assays indicated a significant reduction in cell density and mitochondrial activity, especially at the concentration of 1000 mg/mL of crude extract. The butanol fraction had minimal effects on mitochondrial activity. The wound healing test demonstrated that the biomaterial and extract prevented closure of the wound created in the cell monolayer within 48 h of incubation and caused changes in cell morphology. In view of this, we concluded that a chitosan membrane associated with a 90% ethanol extract of Acmella oleracea exhibited cytotoxic activity is a potential alternative treatment for superficial cancers.-
Descrição: dc.descriptionInstituto de Pesquisa e Desenvolvimento Laboratório de Dinâmica de Compartimentos Celulares Universidade do Vale do Paraíba, SP-
Descrição: dc.descriptionInstituto de Pesquisa e Desenvolvimento Laboratório de Fotobiologia Aplicada à Saúde Universidade do Vale do Paraíba, SP-
Descrição: dc.descriptionLaboratório de Insumos Naturais e Sintéticos Universidade Federal de São Paulo, SP-
Descrição: dc.descriptionUniversidade Estadual de São Paulo Júlio de Mesquita Filho, SP-
Descrição: dc.descriptionUniversidade Estadual de São Paulo Júlio de Mesquita Filho, SP-
Idioma: dc.languageen-
Relação: dc.relationBrazilian Journal of Medical and Biological Research-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBiomaterial-
Palavras-chave: dc.subjectCancer-
Palavras-chave: dc.subjectChitosan-
Palavras-chave: dc.subjectExtract-
Palavras-chave: dc.subjectHEp-2 culture-
Título: dc.titleAntitumor activity of membranes associated with Acmella oleracea extract-
Tipo de arquivo: dc.typelivro digital-
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