Early In Vivo Osteogenic and Inflammatory Response of 3D Printed Polycaprolactone/Carbon Nanotube/Hydroxyapatite/Tricalcium Phosphate Composite Scaffolds

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversity Centre of Hermínio Ometto Foundation-
Autor(es): dc.contributorNanyang Technological University-
Autor(es): dc.contributorThe University of Manchester-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorNalesso, Paulo Roberto Lopes-
Autor(es): dc.creatorVedovatto, Matheus-
Autor(es): dc.creatorGregório, Julia Eduarda Schneider-
Autor(es): dc.creatorHuang, Boyang-
Autor(es): dc.creatorVyas, Cian-
Autor(es): dc.creatorSantamaria-Jr, Milton-
Autor(es): dc.creatorBártolo, Paulo-
Autor(es): dc.creatorCaetano, Guilherme Ferreira-
Data de aceite: dc.date.accessioned2025-08-21T16:17:25Z-
Data de disponibilização: dc.date.available2025-08-21T16:17:25Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-07-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/polym15132952-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/307336-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/307336-
Descrição: dc.descriptionThe development of advanced biomaterials and manufacturing processes to fabricate biologically and mechanically appropriate scaffolds for bone tissue is a significant challenge. Polycaprolactone (PCL) is a biocompatible and degradable polymer used in bone tissue engineering, but it lacks biofunctionalization. Bioceramics, such as hydroxyapatite (HA) and β tricalcium phosphate (β-TCP), which are similar chemically to native bone, can facilitate both osteointegration and osteoinduction whilst improving the biomechanics of a scaffold. Carbon nanotubes (CNTs) display exceptional electrical conductivity and mechanical properties. A major limitation is the understanding of how PCL-based scaffolds containing HA, TCP, and CNTs behave in vivo in a bone regeneration model. The objective of this study was to evaluate the use of three-dimensional (3D) printed PCL-based composite scaffolds containing CNTs, HA, and β-TCP during the initial osteogenic and inflammatory response phase in a critical bone defect rat model. Gene expression related to early osteogenesis, the inflammatory phase, and tissue formation was evaluated using quantitative real-time PCR (RT-qPCR). Tissue formation and mineralization were assessed by histomorphometry. The CNT+HA/TCP group presented higher expression of osteogenic genes after seven days. The CNT+HA and CNT+TCP groups stimulated higher gene expression for tissue formation and mineralization, and pro- and anti-inflammatory genes after 14 and 30 days. Moreover, the CNT+TCP and CNT+HA/TCP groups showed higher gene expressions related to M1 macrophages. The association of CNTs with ceramics at 10wt% (CNT+HA/TCP) showed lower expressions of inflammatory genes and higher osteogenic, presenting a positive impact and balanced cell signaling for early bone formation. The association of CNTs with both ceramics promoted a minor inflammatory response and faster bone tissue formation.-
Descrição: dc.descriptionEngineering and Physical Sciences Research Council-
Descrição: dc.descriptionGraduate Program in Biomedical Sciences University Centre of Hermínio Ometto Foundation, SP-
Descrição: dc.descriptionSingapore Centre for 3D Printing School of Mechanical and Aerospace Engineering Nanyang Technological University, Jurong West-
Descrição: dc.descriptionSchool of Mechanical Aerospace and Civil Engineering The University of Manchester-
Descrição: dc.descriptionGraduate Program of Orthodontics University Centre of Hermínio Ometto Foundation, SP-
Descrição: dc.descriptionDepartment of Social and Pediatric Dentistry UNESP - São Paulo State University Institute of Science and Technology - College of Dentistry, SP-
Descrição: dc.descriptionDivision of Dermatology Department of Internal Medicine Ribeirão Preto Medical School São Paulo University (USP), SP-
Descrição: dc.descriptionDepartment of Social and Pediatric Dentistry UNESP - São Paulo State University Institute of Science and Technology - College of Dentistry, SP-
Idioma: dc.languageen-
Relação: dc.relationPolymers-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subject3D printing-
Palavras-chave: dc.subjectcarbon nanotubes-
Palavras-chave: dc.subjectceramics-
Palavras-chave: dc.subjectcomposites-
Palavras-chave: dc.subjectinflammatory process-
Palavras-chave: dc.subjectosteogenesis-
Palavras-chave: dc.subjecttissue engineering-
Título: dc.titleEarly In Vivo Osteogenic and Inflammatory Response of 3D Printed Polycaprolactone/Carbon Nanotube/Hydroxyapatite/Tricalcium Phosphate Composite Scaffolds-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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