Proteome of amino acids or IGF1-stimulated pacu muscle cells offers molecular insights and suggests FN1B and EIF3C as candidate markers of fish muscle growth

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Federal de Goiás (UFG)-
Autor(es): dc.contributorFederal University of Jataí (UFJ)-
Autor(es): dc.contributorState University of Maringá-
Autor(es): dc.contributorUniversity of Barcelona-
Autor(es): dc.creatorPerez, Erika S.-
Autor(es): dc.creatorRibeiro, Rafaela A.-
Autor(es): dc.creatorZanella, Bruna TT.-
Autor(es): dc.creatorAlmeida, Fernanda LA.-
Autor(es): dc.creatorBlasco, Josefina-
Autor(es): dc.creatorGarcia de la serrana, Daniel-
Autor(es): dc.creatorDal-Pai-Silva, Maeli-
Autor(es): dc.creatorDuran, Bruno OS.-
Data de aceite: dc.date.accessioned2025-08-21T19:59:03Z-
Data de disponibilização: dc.date.available2025-08-21T19:59:03Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2025-04-09-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.bbrc.2025.151648-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/307291-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/307291-
Descrição: dc.descriptionStudy of fish skeletal muscle is essential to understand physiological or metabolic processes, and to develop programs searching for increased muscle mass and meat production. Amino acids (AA) and IGF1 stimulate processes that lead to muscle growth, but their signaling pathways and molecular regulation need further clarification in fish. We obtained the proteome of pacu (Piaractus mesopotamicus) cultured muscle cells treated with AA or IGF1, which induced the differential abundance of 67 and 53 proteins, respectively. Enrichment analyses showed that AA modulated histone methylation, cell differentiation, and metabolism, while IGF1 modulated ATP production and protein synthesis. In addition, we identified molecular networks with candidate markers that commonly regulate fish muscle cells: FN1B and EIF3C, respectively up- and down-regulated by both treatments. FN1B was related to cell proliferation, protein synthesis, and muscle repair, while EIF3C connected with negative regulators of muscle growth. Their gene expression was evaluated in pacu and Nile tilapia (Oreochromis niloticus) after nutrient manipulation, with fn1b increased during refeeding and eif3c increased during fasting in both species. Our work helps clarify the molecular regulation by AA or IGF1 and suggests that FN1B and EIF3C could be potential stimulatory and inhibitory biomarkers of fish muscle growth.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionDepartment of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionDepartment of Histology Embryology and Cell Biology Institute of Biological Sciences Federal University of Goiás (UFG), Goiás-
Descrição: dc.descriptionDepartment of Morphophysiology Institute of Biosciences Federal University of Jataí (UFJ), Goiás-
Descrição: dc.descriptionDepartment of Morphological Sciences Center of Biological Sciences State University of Maringá, Paraná-
Descrição: dc.descriptionDepartment of Cell Biology Physiology and Immunology Faculty of Biology University of Barcelona-
Descrição: dc.descriptionDepartment of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionFAPESP: #2018/24575-6-
Descrição: dc.descriptionFAPESP: #2018/26428-0-
Descrição: dc.descriptionCNPq: #306678/2021-7-
Descrição: dc.descriptionCNPq: #403305/2021-7-
Idioma: dc.languageen-
Relação: dc.relationBiochemical and Biophysical Research Communications-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBioinformatics-
Palavras-chave: dc.subjectCell culture-
Palavras-chave: dc.subjectFish-
Palavras-chave: dc.subjectProteome-
Palavras-chave: dc.subjectSkeletal muscle-
Título: dc.titleProteome of amino acids or IGF1-stimulated pacu muscle cells offers molecular insights and suggests FN1B and EIF3C as candidate markers of fish muscle growth-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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