Neem Essential Oil as an Antifungal Agent against Phyllosticta citricarpa

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorSanta Catarina State University (UDESC)-
Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.creatorSchuch, Joyce Maria-
Autor(es): dc.creatorMendes, Carolina Rosai-
Autor(es): dc.creatorCardoso, Guilherme Lopes-
Autor(es): dc.creatorAndré Da Veiga Lima Rosa Costamilan, Carlos-
Autor(es): dc.creatorMatos Lopes, Paulo Renato-
Autor(es): dc.creatorMontagnolli, Renato Nallin-
Autor(es): dc.creatorDilarri, Guilherme-
Autor(es): dc.creatorBidoia, Ederio Dino-
Data de aceite: dc.date.accessioned2025-08-21T22:18:56Z-
Data de disponibilização: dc.date.available2025-08-21T22:18:56Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1155/2024/6251407-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/307240-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/307240-
Descrição: dc.descriptionThe fungus Phyllosticta citricarpa is a quarantine phytopathogen responsible for causing citrus black spot (CBS) disease. To export fruits to CBS-free countries, they must undergo a sanitation process to ensure disease control. In this study, neem essential oil (NEO) was tested against P. citricarpa for the first time as an alternative sanitizer. In vitro experiments were conducted to determine the inhibition concentration of NEO for P. citricarpa, and the mode of action of the essential oil was evaluated. In vivo assays were performed to simulate the sanitization process used in packinghouses. NEO was characterized by GC-MS/MS. The results revealed that NEO at 100 μL·mL-1 exhibited a similar inhibitory effect as copper oxychloride, suppressing 89.68 ± 1.14% of fungal mycelium growth. Fluorescence microscopy experiments demonstrated that NEO functions by disrupting the cytoplasmic membrane of fungal hyphae, leading to their death within 30 minutes of contact with NEO. GC-MS/MS characterization revealed a high presence of phenolic compounds, which serve as the primary antifungal agents responsible for the action against fungal hyphae. In vivo assays showed that NEO at 100 μL·mL-1 also reduced microorganisms (CFU mL-1) by 93.00 ± 3.88% compared to the negative control. Overall, the results demonstrate that NEO can effectively serve as an alternative sanitizer against P. citricarpa in citrus packinghouses. Our findings allow future studies to explore the use of NEO for sanitizing other fruits and combating different phytopathogens to broaden its potential application in fruit sanitation for export.-
Descrição: dc.descriptionDepartment of General and Applied Biology Sao Paulo State University (UNESP), Avenida 24-A 1515, SP-
Descrição: dc.descriptionDepartment of Fisheries Engineering and Biological Sciences Santa Catarina State University (UDESC), Rua Coronel Fernandes Martins 270, SC-
Descrição: dc.descriptionCollege of Technology and Agricultural Sciences Sao Paulo State University (UNESP), SP-294 Km 651, SP-
Descrição: dc.descriptionDepartment of Natural Sciences Mathematics and Education Federal University of Sao Carlos (UFSCar), SP-330 Km 174, SP-
Descrição: dc.descriptionMulticentric Graduate Program in Biochemistry and Molecular Biology (PMBqBM) Santa Catarina State University (UDESC), Avenida Luiz de Camões 2090, SC-
Descrição: dc.descriptionDepartment of General and Applied Biology Sao Paulo State University (UNESP), Avenida 24-A 1515, SP-
Descrição: dc.descriptionCollege of Technology and Agricultural Sciences Sao Paulo State University (UNESP), SP-294 Km 651, SP-
Idioma: dc.languageen-
Relação: dc.relationInternational Journal of Microbiology-
???dc.source???: dc.sourceScopus-
Título: dc.titleNeem Essential Oil as an Antifungal Agent against Phyllosticta citricarpa-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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