Effect of laser photobiomodulation combined with hydroxyapatite nanoparticles on the osteogenic differentiation of mesenchymal stem cells using artificial intelligence: An in vitro study

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Autor(es): dc.contributorUniversity of Pernambuco-
Autor(es): dc.contributorUniversidade Federal de Pernambuco (UFPE)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorde Melo, Eloiza Leonardo-
Autor(es): dc.creatorMiranda, Jéssica Meirinhos-
Autor(es): dc.creatorde Souza Rolim Lima, Vanessa Bastos-
Autor(es): dc.creatorGaião, Wyndly Daniel Cardoso-
Autor(es): dc.creatorde Vilhena Amorim Tostes, Braulio-
Autor(es): dc.creatorRodrigues, Claudio Gabriel-
Autor(es): dc.creatorda Silva, Márcia Bezerra-
Autor(es): dc.creatorJúnior, Severino Alves-
Autor(es): dc.creatorPerger, Edson Luiz Pontes-
Autor(es): dc.creatorBispo, Mávio Eduardo Azevedo-
Autor(es): dc.creatorde Martínez Gerbi, Marleny Elizabeth Márquez-
Data de aceite: dc.date.accessioned2025-08-21T20:43:39Z-
Data de disponibilização: dc.date.available2025-08-21T20:43:39Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-10-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0313787-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/307221-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/307221-
Descrição: dc.descriptionAim To evaluate in vitro the effect of laser photobiomodulation (PBM) combined or not with 30-nm hydroxyapatite nanoparticles (HANp), on the osteogenic differentiation of human umbilical cord mesenchymal stem cells (hUC-MSCs) by morphometric analysis using artificial intelligence programs (TensorFlow and ArcGIS). Methods UC-MSCs were isolated and cultured until 80% confluence was reached. The cells were then plated according to the following experimental groups: G1 –control (DMEM), G2 – BMP-2, G3 –BMP-7, G4 –PBM (660 nm, 10 mW, 2.5 J/cm2, spot size of 0.08 cm2), G5 – HANp, G6 –HANp + PBM, G7 –BMP-2 + PBM, and G8 –BMP-7 + PBM. The MTT assay was used to analyze cell viability at 24, 48 and 72 h. Osteogenic differentiation was assessed by Alizarin Red staining after 7, 14 and 21 days. For morphometric analysis, areas of osteogenic differentiation (pixel2) were delimited by machine learning using the TensorFlow and ArcGIS 10.8 programs. Results The results of the MTT assay showed high rates of cell viability and proliferation in all groups when compared to control. Morphometric analysis revealed a greater area of osteogenic differentiation in G5 (HANp = 142709,33±36573,39) and G6 (HANp + PBM = 125452,00 ±24226,95) at all time points evaluated. Conclusion It is suggested that HANp, whether combined with PBM or not, may be a promising alternative to enhance the cellular viability and osteogenic differentiation of hUC-MSCs.-
Descrição: dc.descriptionDepartment of Biophotonics in Health Sciences University of Pernambuco, Pernambuco-
Descrição: dc.descriptionDepartment of Biophysics and Radiobiology Federal University of Pernambuco, Pernambuco-
Descrição: dc.descriptionDepartment of Basic Chemistry Federal University of Pernambuco, Pernambuco-
Descrição: dc.descriptionDepartment of Bioprocess and Biotechnology Paulista State University, Botucatu-
Descrição: dc.descriptionDepartment of Computer Science University of Pernambuco, Pernambuco-
Descrição: dc.descriptionDepartment of Bioprocess and Biotechnology Paulista State University, Botucatu-
Idioma: dc.languageen-
Relação: dc.relationPLoS ONE-
???dc.source???: dc.sourceScopus-
Título: dc.titleEffect of laser photobiomodulation combined with hydroxyapatite nanoparticles on the osteogenic differentiation of mesenchymal stem cells using artificial intelligence: An in vitro study-
Tipo de arquivo: dc.typelivro digital-
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