Molecular and Physiological Effects of 17α-methyltestosterone on Sex Differentiation of Black Rockfish, Sebastes schlegelii

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Autor(es): dc.contributorShanghai Ocean University-
Autor(es): dc.contributorChinese Academy of Fishery Sciences-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorNingbo University-
Autor(es): dc.contributorQingdao Marine Science and Technology Center-
Autor(es): dc.creatorHuang, Haijun-
Autor(es): dc.creatorLiu, Yuyan-
Autor(es): dc.creatorWang, Qian-
Autor(es): dc.creatorDong, Caichao-
Autor(es): dc.creatorDong, Le-
Autor(es): dc.creatorZhang, Jingjing-
Autor(es): dc.creatorYang, Yu-
Autor(es): dc.creatorHao, Xiancai-
Autor(es): dc.creatorLi, Weijing-
Autor(es): dc.creatorRosa, Ivana F.-
Autor(es): dc.creatorDoretto, Lucas B.-
Autor(es): dc.creatorCao, Xuebin-
Autor(es): dc.creatorShao, Changwei-
Data de aceite: dc.date.accessioned2025-08-21T20:24:13Z-
Data de disponibilização: dc.date.available2025-08-21T20:24:13Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-05-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/genes15050605-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/307121-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/307121-
Descrição: dc.descriptionIt is widely known that all-female fish production holds economic value for aquaculture. Sebastes schlegelii, a preeminent economic species, exhibits a sex dimorphism, with females surpassing males in growth. In this regard, achieving all-female black rockfish production could significantly enhance breeding profitability. In this study, we utilized the widely used male sex-regulating hormone, 17α-methyltestosterone (MT) at three different concentrations (20, 40, and 60 ppm), to produce pseudomales of S. schlegelii for subsequent all-female offspring breeding. Long-term MT administration severely inhibits the growth of S. schlegelii, while short term had no significant impact. Histological analysis confirmed sex reversal at all MT concentrations; however, both medium and higher MT concentrations impaired testis development. MT also influenced sex steroid hormone levels in pseudomales, suppressing E2 while increasing T and 11-KT levels. In addition, a transcriptome analysis revealed that MT down-regulated ovarian-related genes (cyp19a1a and foxl2) while up-regulating male-related genes (amh) in pseudomales. Furthermore, MT modulated the TGF-β signaling and steroid hormone biosynthesis pathways, indicating its crucial role in S. schlegelii sex differentiation. Therefore, the current study provides a method for achieving sexual reversal using MT in S. schlegelii and offers an initial insight into the underlying mechanism of sexual reversal in this species.-
Descrição: dc.descriptionNational Key Research and Development Program of China-
Descrição: dc.descriptionCentral Public-interest Scientific Institution Basal Research Fund, Chinese Academy of Fishery Sciences-
Descrição: dc.descriptionCollege of Fisheries and Life Science Shanghai Ocean University-
Descrição: dc.descriptionState Key Laboratory of Mariculture Biobreeding and Sustainable Goods Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences-
Descrição: dc.descriptionDepartment of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP-
Descrição: dc.descriptionSchool of Marine Sciences Ningbo University-
Descrição: dc.descriptionLaboratory for Marine Fisheries Science and Food Production Processes Qingdao Marine Science and Technology Center-
Descrição: dc.descriptionDepartment of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP-
Descrição: dc.descriptionNational Key Research and Development Program of China: 2022YFD2400100-
Descrição: dc.descriptionCentral Public-interest Scientific Institution Basal Research Fund, Chinese Academy of Fishery Sciences: 2023TD19-
Descrição: dc.descriptionCentral Public-interest Scientific Institution Basal Research Fund, Chinese Academy of Fishery Sciences: 2023XT0203-
Descrição: dc.descriptionCentral Public-interest Scientific Institution Basal Research Fund, Chinese Academy of Fishery Sciences: 20603022023008-
Idioma: dc.languageen-
Relação: dc.relationGenes-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subject17α-methyltestosterone-
Palavras-chave: dc.subjectgonadal development-
Palavras-chave: dc.subjectS. schlegelii-
Palavras-chave: dc.subjectsex hormone-
Palavras-chave: dc.subjectsex reversal-
Palavras-chave: dc.subjecttranscriptomics-
Título: dc.titleMolecular and Physiological Effects of 17α-methyltestosterone on Sex Differentiation of Black Rockfish, Sebastes schlegelii-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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