DNA methylation-based depiction of the immune microenvironment and immune-associated long non-coding RNAs in oral cavity squamous cell carcinomas

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversity of Southern Denmark-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorA.C.Camargo Cancer Center-
Autor(es): dc.contributorMcGill University-
Autor(es): dc.contributorSENAI CIMATEC-
Autor(es): dc.contributorFIOCRUZ-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorCalanca, Naiade-
Autor(es): dc.creatorFrancisco, Ana Lucia Noronha-
Autor(es): dc.creatorBizinelli, Daniela-
Autor(es): dc.creatorKuasne, Hellen-
Autor(es): dc.creatorBarros Filho, Mateus Camargo-
Autor(es): dc.creatorFlores, Bianca Campos Troncarelli-
Autor(es): dc.creatorPinto, Clóvis Antonio Lopes-
Autor(es): dc.creatorRainho, Claudia Aparecida-
Autor(es): dc.creatorSoares, Milena Botelho Pereira-
Autor(es): dc.creatorMarchi, Fabio Albuquerque-
Autor(es): dc.creatorKowalski, Luiz Paulo-
Autor(es): dc.creatorRogatto, Silvia Regina-
Data de aceite: dc.date.accessioned2025-08-21T16:12:26Z-
Data de disponibilização: dc.date.available2025-08-21T16:12:26Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-10-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.biopha.2023.115559-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/307046-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/307046-
Descrição: dc.descriptionOral cavity squamous cell carcinoma (OSCC) is a complex and dynamic disease characterized by clinicopathological and molecular heterogeneity. Spatial and temporal heterogeneity of cell subpopulations has been associated with cancer progression and implicated in the prognosis and therapy response. Emerging evidence indicates that aberrant epigenetic profiles in OSCC may foster an immunosuppressive tumor microenvironment by modulating the expression of immune-related long non-coding RNAs (lncRNAs). DNA methylation analysis was performed in 46 matched OSCC and normal adjacent tissue samples using a genome-wide platform (Infinium HumanMethylation450 BeadChip). Reference-based computational deconvolution (MethylCIBERSORT) was applied to infer the immune cell composition of the bulk samples. The expression levels of genes encoding immune markers and differentially methylated lncRNAs were investigated using The Cancer Genome Atlas dataset. OSCC specimens presented distinct immune cell composition, including the enrichment of monocyte lineage cells, natural killer cells, cytotoxic T-lymphocytes, regulatory T-lymphocytes, and neutrophils. In contrast, B-lymphocytes, effector T-lymphocytes, and fibroblasts were diminished in tumor samples. The hypomethylation of three immune-associated lncRNAs (MEG3, MIR155HG, and WFDC21P) at individual CpG sites was confirmed by bisulfite-pyrosequencing. Also, the upregulation of a set of immune markers (FOXP3, GZMB, IL10, IL2RA, TGFB, IFNG, TDO2, IDO1, and HIF1A) was detected. The immune cell composition, immune markers alteration, and dysregulation of immune-associated lncRNAs reinforce the impact of the immune microenvironment in OSCC. These concurrent factors contribute to tumor heterogeneity, suggesting that epi-immunotherapy could be an efficient alternative to treat OSCC.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionDepartment of Clinical Genetics University Hospital of Southern Denmark-Vejle Institute of Regional Health Research University of Southern Denmark-
Descrição: dc.descriptionDepartment of Chemical and Biological Sciences Institute of Biosciences São Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Head and Neck Surgery and Otorhinolaryngology A.C.Camargo Cancer Center, SP-
Descrição: dc.descriptionInternational Research Center (CIPE) A.C.Camargo Cancer Center, SP-
Descrição: dc.descriptionRosalind and Morris Goodman Cancer Institute McGill University-
Descrição: dc.descriptionDepartment of Pathology A.C.Camargo Cancer Center, SP-
Descrição: dc.descriptionHealth Technology Institute SENAI CIMATEC, BA-
Descrição: dc.descriptionGonçalo Moniz Institute FIOCRUZ, BA-
Descrição: dc.descriptionDepartment of Head and Neck Surgery University of São Paulo Medical School, SP-
Descrição: dc.descriptionDepartment of Chemical and Biological Sciences Institute of Biosciences São Paulo State University (UNESP), SP-
Descrição: dc.descriptionFAPESP: 2008/57887–9-
Descrição: dc.descriptionCNPq: 573589/08–9-
Descrição: dc.descriptionCAPES: 88887.310463/2018-00-
Idioma: dc.languageen-
Relação: dc.relationBiomedicine and Pharmacotherapy-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectDeconvolution-
Palavras-chave: dc.subjectDNA methylation-
Palavras-chave: dc.subjectLncRNAs-
Palavras-chave: dc.subjectOral cancer-
Palavras-chave: dc.subjectTumor immune microenvironment-
Título: dc.titleDNA methylation-based depiction of the immune microenvironment and immune-associated long non-coding RNAs in oral cavity squamous cell carcinomas-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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