Citric Acid Conditioning as an Alternative to EDTA for Growth Factors Release and Stem Cell Response in Regenerative Endodontics: A Systematic Review of In Vitro Studies

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Autor(es): dc.contributorUniversidade Federal de Minas Gerais (UFMG)-
Autor(es): dc.contributorSchool of Dentistry-
Autor(es): dc.contributorFaculty of Dental Medicine-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorReis-Prado, A.H. dos-
Autor(es): dc.creatorToledo, P. T.A.-
Autor(es): dc.creatorNunes, G. P.-
Autor(es): dc.creatorFerreira, P. A.V.-
Autor(es): dc.creatorRahimnejad, M.-
Autor(es): dc.creatorDal-Fabbro, R.-
Autor(es): dc.creatorAbreu, L. G.-
Autor(es): dc.creatorBottino, M. C.-
Autor(es): dc.creatorBenetti, F.-
Data de aceite: dc.date.accessioned2025-08-21T16:12:38Z-
Data de disponibilização: dc.date.available2025-08-21T16:12:38Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-01-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.joen.2023.11.006-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/307004-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/307004-
Descrição: dc.descriptionIntroduction: Citric acid (CA) conditioning may be a promising alternative to ethylenediaminetetraacetic acid (EDTA) in regenerative endodontic procedures, as reported to improve growth factors’ release from dentin. This review systematically investigated the effect of CA conditioning on the growth factors release from dentin and cell behavior compared to EDTA conditioning. Methods: Searches were conducted (PubMed/MEDLINE, Scopus, Web of Science, Embase, SciELO, Cochrane Library, and grey literature) until May-2023. Only in vitro studies that evaluated the effects of CA on growth factors' release from dentin and cell behavior outcomes compared to EDTA were included. The studies were critically appraised using a modified Joanna Briggs Institute's checklist. Meta-analysis was unfeasible. Results: Out of the 335 articles screened, nine were included. Among these, three studies used dentin discs/roots from permanent human teeth; the rest combined them with stem cells. 10% CA for 5 or 10 minute was the most used protocol. Meanwhile, EDTA concentrations ranged from 10% to 17%. In eight studies examining the release of growth factors, five reported a significant release of transforming growth factor-β after dentin conditioning with 10% CA compared to 17% EDTA. Regarding cell behavior (6 studies), three studies assessed cell viability. The findings revealed that 10% CA conditioning showed cell viability similar to those of 17% EDTA. Additionally, in two out of three studies, it was observed that 10% CA conditioning did not affect cell morphology. The studies had a low risk of bias. Conclusions: The use of 10% CA to condition dentin for 5-10 minutes resulted in a notable transforming growth factor -β1 release, but its cell responses were similar to those of EDTA.-
Descrição: dc.descriptionRestorative Dentistry Universidade Federal de Minas Gerais (UFMG) School of Dentistry, Minas Gerais-
Descrição: dc.descriptionCariology Restorative Sciences and Endodontics University of Michigan School of Dentistry-
Descrição: dc.descriptionLaboratory for Bone Metabolism and Regeneration University of Porto Faculty of Dental Medicine-
Descrição: dc.descriptionPreventive and Restorative Dentistry São Paulo State University (Unesp) School of Dentistry, São Paulo-
Descrição: dc.descriptionChild's and Adolescent's Oral Health Universidade Federal de Minas Gerais (UFMG) School of Dentistry, Minas Gerais-
Descrição: dc.descriptionPreventive and Restorative Dentistry São Paulo State University (Unesp) School of Dentistry, São Paulo-
Formato: dc.format129-143-
Idioma: dc.languageen-
Relação: dc.relationJournal of Endodontics-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCitric acid-
Palavras-chave: dc.subjectEDTA-
Palavras-chave: dc.subjectgrowth factor(s)-
Palavras-chave: dc.subjectregenerative endodontics-
Palavras-chave: dc.subjectstem cells-
Palavras-chave: dc.subjectsystematic review-
Título: dc.titleCitric Acid Conditioning as an Alternative to EDTA for Growth Factors Release and Stem Cell Response in Regenerative Endodontics: A Systematic Review of In Vitro Studies-
Tipo de arquivo: dc.typevídeo-
Aparece nas coleções:Repositório Institucional - Unesp

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