Direct assessment of leukocyte signalling and cytokine secretion reveals exercise intensity-dependent reductions in anti-inflammatory cytokine action

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversity of British Columbia Okanagan-
Autor(es): dc.contributorNational Taiwan Normal University-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversity of Castilla-La Mancha-
Autor(es): dc.contributorUniversity of Coimbra-
Autor(es): dc.creatorIslam, Hashim-
Autor(es): dc.creatorTsai, Shun-Hsi-
Autor(es): dc.creatorFigueiredo, Caíque-
Autor(es): dc.creatorJackson, Garett S.-
Autor(es): dc.creatorMarcotte-Chénard, Alexis-
Autor(es): dc.creatorBosak, Johannes-
Autor(es): dc.creatorMoreno-Cabañas, Alfonso-
Autor(es): dc.creatorLira, Fabio S.-
Autor(es): dc.creatorLittle, Jonathan P.-
Data de aceite: dc.date.accessioned2025-08-21T21:27:39Z-
Data de disponibilização: dc.date.available2025-08-21T21:27:39Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-06-15-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1113/JP286228-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/306731-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/306731-
Descrição: dc.descriptionAbstract: Circulating interleukin (IL)-6 and IL-10 concentrations are widely used to evaluate the anti-inflammatory effects of exercise but do not capture cytokine action at the cellular level. Whether and how acute exercise impacts anti-inflammatory cytokine action in humans is unknown. To determine how exercise intensity and pattern impact IL-6 and IL-10 action in blood leukocytes, 16 active adults (eight males/eight females; age: 30 ± 3 years; body mass index: 22.8 ± 2.3 kg/m2; (Formula presented.) : 51 ± 6 mL/kg/min) completed a no-exercise control condition (CTL) or isocaloric bouts of cycling performed below (moderate continuous exercise; MCE) or above (heavy continuous or heavy intermittent exercise; HCE or HIE, respectively) lactate threshold. Venous blood (before, after, 30 min after and 90 min after exercise) was analysed for immune cell subpopulations, plasma cytokine concentrations, anti-inflammatory cytokine action and monocyte phenotype. Exercise induced rapid leukocytosis (P < 0.001) and increased plasma IL-6 (P < 0.001), IL-10 (P = 0.0145) and tumour necrosis factor-⍺ (TNF-⍺) (P = 0.0338) concentrations in an intensity-dependent manner (HCE and/or HIE vs. CTL). These systemic changes coincided with a diminished ability of IL-10/6 to phosphorylate STAT3 (P < 0.001) and inhibit TNF-⍺ secretion (P = 0.0238) in blood leukocytes following HCE and HIE. Monocyte polarization experiments revealed lower CD80 [MCE (P = 0.0933) and HIE (P = 0.0187) vs. CTL] and a tendency for higher CD163 expression (HCE vs. CTL, P = 0.0985), suggesting that hyporesponsiveness to anti-inflammatory cytokine action does not impede the ability of exercise to promote an anti-inflammatory monocyte phenotype. These findings provide novel insights into the immunomodulatory effects of exercise in humans and highlight the importance of directly measuring cellular cytokine action when evaluating the anti-inflammatory effects of exercise. (Figure presented.). Key points: Circulating cytokine concentrations are frequently used to evaluate the anti-inflammatory effects of exercise but may not capture changes in cytokine action occurring at the cellular level. We directly assessed anti-inflammatory cytokine action – measured using a combination of intracellular signalling and cytokine secretion ex vivo – in distinct immune cell subpopulations after acute calorie-matched exercise bouts differing in intensity and pattern. Anti-inflammatory cytokine action was blunted following higher intensity exercise despite corresponding increases in circulating cytokine concentrations and immune cell counts. Changes in cytokine action were not explained by changes in cytokine receptor expression on circulating immune cells. Our findings provide new insights into the immunomodulatory effects of exercise in humans and highlight the importance of directly measuring cellular cytokine action when evaluating the anti-inflammatory effects of exercise.-
Descrição: dc.descriptionSchool of Health and Exercise Sciences University of British Columbia Okanagan-
Descrição: dc.descriptionDepartment of Physical Education and Sport Sciences National Taiwan Normal University-
Descrição: dc.descriptionExercise and Immunometabolism Research Group Department of Physical Education Post-Graduation Program in Movement Sciences State University of São Paulo (UNESP), São Paulo-
Descrição: dc.descriptionExercise Physiology Lab at Toledo University of Castilla-La Mancha-
Descrição: dc.descriptionCentro de Investigação em Desporto e Atividade Física University of Coimbra-
Descrição: dc.descriptionExercise and Immunometabolism Research Group Department of Physical Education Post-Graduation Program in Movement Sciences State University of São Paulo (UNESP), São Paulo-
Formato: dc.format2717-2736-
Idioma: dc.languageen-
Relação: dc.relationJournal of Physiology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectIL-10-
Palavras-chave: dc.subjectIL-6-
Palavras-chave: dc.subjectimmunometabolism-
Palavras-chave: dc.subjectinflammation-
Palavras-chave: dc.subjectmonocyte-
Palavras-chave: dc.subjectSTAT3-
Palavras-chave: dc.subjectTNF-⍺-
Título: dc.titleDirect assessment of leukocyte signalling and cytokine secretion reveals exercise intensity-dependent reductions in anti-inflammatory cytokine action-
Tipo de arquivo: dc.typelivro digital-
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