Escherichia coli as a sentinel in the assessment of antimicrobial resistance in the tilapia production chain: from production environment to the final product

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Federal de Uberlândia (UFU)-
Autor(es): dc.contributorUniversidade Federal de Viçosa (UFV)-
Autor(es): dc.contributorUniversidade Federal do Paraná (UFPR)-
Autor(es): dc.creatorDias, Sthéfany Da Cunha-
Autor(es): dc.creatorCosta, Letícia Roberta Martins-
Autor(es): dc.creatorBuiatte, Ana Beatriz Garcez-
Autor(es): dc.creatorCossi, Marcus Vinícius Coutinho-
Autor(es): dc.creatorNero, Luís Augusto-
Autor(es): dc.creatorYamatogi, Ricardo Seiti-
Autor(es): dc.creatorBersot, Luciano dos Santos-
Autor(es): dc.creatorPereira, Juliano Gonçalves-
Data de aceite: dc.date.accessioned2025-08-21T17:08:27Z-
Data de disponibilização: dc.date.available2025-08-21T17:08:27Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3389/frabi.2024.1461662-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/306703-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/306703-
Descrição: dc.descriptionIntroduction: The intensification of tilapia production has increased animal density in tanks, leading to more frequent exposure to pathogenic agents and compromising the quality of fish products. Antimicrobial resistance is a global concern that affects human treatment, and sentinel microorganisms like Escherichia coli are crucial for monitoring production chains, especially in aquaculture, where research is still limited. The aim of this study was to identify the presence of E. coli and investigate its antimicrobial resistance profiles throughout the entire tilapia production chain. Methods: A total of 240 samples were collected from various points in the production process: carcasses before scaling (Ca), scaling wastewater (Sw), filleting wastewater (Fw), fillet washing wastewater (Tw), fillet handling surfaces (Su), and pre-packaged fillets (Pf). The samples were collected during 10 visits, each corresponding to animals from different farms. E. coli isolates were identified using MacConkey agar and biochemical tests. Phenotypic resistance profiles were determined using nine classes of antimicrobials. Extended- spectrum b-lactamase (ESBL) production was identified with ceftazidime and cefotaxime and confirmed by a double-disc synergy test. Isolates were classified as sensitive or resistant based on the inhibition zone. Multidrug-resistant (MDR) was defined as resistance to at least one agent in three or more antimicrobial categories, while extensively drug-resistant (XDR) was defined as resistance to at least one agent in all but two or fewer categories. Results: Overall, 50.8% of the samples (122/240) tested positive for E. coli, with 403 isolates identified. Of these, 33% (133/403) were resistant to at least two antimicrobials, and 20% (48/240) of the samples had MDR isolates, with the highest frequency found at the filleting point (Fw), which also had the only XDR profile. Resistance was most commonly observed against amoxicillin (35.73%), tetracycline (30.77%), and ciprofloxacin (26.30%). Discussion: These findings emphasize the importance of E. coli as an indicator of antimicrobial resistance throughout tilapia processing and highlight the need for good production practices and qualified technical support to mitigate risks to public health, animal health, and the environment.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionDepartment of Animal Production and Preventive Veterinary Medicine São Paulo State University Júlio de Mesquita Filho (UNESP), São Paulo-
Descrição: dc.descriptionFaculty of Veterinary Medicine Federal University of Uberlândia (UFU), Minas Gerais-
Descrição: dc.descriptionDepartment of Veterinary Medicine Federal University of Viçosa (UFV)-
Descrição: dc.descriptionDepartment of Veterinary Sciences Federal University of Paraná (UFPR)-
Descrição: dc.descriptionDepartment of Animal Production and Preventive Veterinary Medicine São Paulo State University Júlio de Mesquita Filho (UNESP), São Paulo-
Idioma: dc.languageen-
Relação: dc.relationFrontiers in Antibiotics-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectantibiotics-
Palavras-chave: dc.subjectE. coli-
Palavras-chave: dc.subjectmicrobial sensitivity-
Palavras-chave: dc.subjectresistance-
Palavras-chave: dc.subjecttilapia-
Título: dc.titleEscherichia coli as a sentinel in the assessment of antimicrobial resistance in the tilapia production chain: from production environment to the final product-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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